肺炎链球菌疫苗抗原促进CRTH2(CD4^+CD294^+Th2)细胞增殖的实验研究  

Pneumococcal vaccine promotes the proliferation of CRTH2 (CD4^+CD294^+Th2) cells

作　　者：唐玫琴[1] 田发青[1] 李举亨[1] 黄映彩 李惠卿[1] 成小慧 TANG Mei-qin TIAN Fa-qing LI Ju-heng HUANG Ying-cai LI Hui-qing CHENG Xiao- hui(Longgang District People ＇s Hospital of Shenzhen, Shenzhen 518172, China)

机构地区：[1]深圳市龙岗区人民医院,广东深圳518172

出　　处：《中国感染控制杂志》2017年第10期916-919,930,共5页Chinese Journal of Infection Control

基　　金：深圳市科技创新项目科技研发基金(JCYI 20140414123738256);深圳市龙岗区科技发展基金(YLWS20150514150041453)

摘　　要：目的研究肺炎链球菌疫苗通过树突状细胞抗原递呈作用对CRTH2(CD4^+CD294^+Th2)细胞促增殖作用,为扩增及分选Th2细胞提供新方法。方法外周血单核细胞培养为树突状细胞,负载肺炎链球菌疫苗抗原后与T淋巴细胞共培养,CCK8法测定混合淋巴细胞反应,流式细胞仪分析DC及CRTH2细胞。结果肺炎链球菌疫苗可促进树突状细胞成熟,与TNF-a共同是DC成熟佐剂。DC负载肺炎链球菌疫苗抗原能使第5天CRTH2细胞亚群比率[(0.93±0.10)%]较首日[(0.70±0.02)%]升高,其绝对数也升高(均P<0.05)。结论树突状细胞负载肺炎链球菌疫苗抗原能够促进CRTH2细胞增殖,可能是扩增Th2细胞有效方法之一。Objective To study the proliferation of CRTH2 （CD4＋ CD294＊ Th2） cells promoted by pneumococcal vaccine through antigen presentation of dendritic cells （DCs）, so as to provide a new approach for amplification and sorting of Th2 cells. Methods CDs induced from peripheral blood mononuclear cells were cocultured with T lym- phocytes after loading pneumococcal vaccine antigen, mixed lymphocyte reaction （MLR） was detected by cell count- ing kit-8（CCK8）, DCs and CRTH2 cells were analyzed by flow eytometry. Results Pneumococcal vaccine could promote the maturation of DCs, together with TNF-a, it was adjuvant for maturation of DCs. Pneumococcal vaccine antigen-loaded DCs could increase the rate of subsets of CRTH2 cells on day 5（[0.93 ± 0.10] % ） compared with day 1（[0.70 ± 0.02] % ）, and absolute number also increased （both P〈0.05）. Conclusion Amplification of CRTH2 cells can be greatly promoted by pneumocoecal vaccine antigen-loaded DCs, which might be one of the effective way to induce amplification of Th2 cells.

关 键 词：肺炎链球菌疫苗 树突状细胞 CRTH2细胞 细胞增殖 

分 类 号：R392.12[医药卫生—免疫学]