马钱子碱微囊制备工艺的研究  

Study on the preparation technology of brucine microcapsules

作　　者：张少孩 王鸿[2] 尚小广[2] 

机构地区：[1]浙江中医药大学药学系,浙江杭州310053 [2]杭州医学院,浙江杭州310053

出　　处：《中国现代医生》2017年第27期42-45,F0003,共5页China Modern Doctor

基　　金：浙江省卫计委科研计划(2010QNA002);浙江医学高等专科学校(现杭州医学院)科研基金项目(2015XZA01)

摘　　要：目的制备马钱子碱微囊,考察其影响因素,找出最优方案。方法以壳聚糖和明胶为囊材,马钱子碱为囊心物,采用复凝聚法制备载药微囊。通过正交设计试验,以包封率、粒径为指标,确定最佳囊材比、囊心与囊材比及CaCl_2的浓度,确定最优配方。观察其形态,测定其包封率、粒径、载药量、Zeta电位等。结果通过正交实验,制备马钱子碱微囊的最优配方:壳聚糖浓度为5 mg/m L,明胶浓度为4 mg/m L,CaCl_2浓度为2%,囊心与囊材比为1∶3。3次平行制备所得的载药微囊的各参数的平均值:包封率为28.73%,粒径为2.15μm,载药量为10.6%,Zeta电位为12.61 mV。微囊分散性好,表面平滑完整,囊壁清晰,无粘连。结论以壳聚糖和明胶为原料的复凝聚法制备马钱子碱微囊具有较高的包封率,且稳定性好,制备工艺简单。Objective To prepare brucine microcapsules, investigate the influencing factors and find out the optimal scheme. Methods Chitosan and gelatin were used as the capsule materials,and brucine was taken as the core materials. Drug-loaded microcapsules were prepared by complex coagulation method. Through orthogonal design test, with the encapsulation efficiency and particle size as an indicator, the best ratio of capsule materials, ratio of core to materials and the concentration of CaC12 were determined, and the optimal formula was determined. Its shape was observed, and the encapsulation efficiency, particle size, drug loading capacity and zeta potential were measured. Results Through orthogonal design test,the optimum formula for preparing brucine microcapsules was as follows： The concentration of chitosan was 5 mg/mL, gelatin concentration 4 mg/mL, CaCX2 concentration 2%, and the ratio of capsule core to capsule materials was 1：3. The average values of the parameters of the drug-loaded microcapsules prepared in three parallel preparations： The encapsulation efficiency was 28.73%, particle size 2.15μm, the drug loading capacity 10.6% and Zeta potential 12.61 inV. Microcapsules were with good dispersion, smooth and complete surface, clear wall, and no adhesion. Conclusion The preparation of brucine microcapsules with chitosan and gelatin as raw materials has a high encapsulation efficiency, and the stability is good and the preparation process is simple.

关 键 词：马钱子碱 微囊 壳聚糖 明胶 

分 类 号：R917.4[医药卫生—药物分析学]