福建省动物宿主感染巴贝虫调查研究  

Natural hosts infected with Babesiain Fujian Province,China

作　　者：肖方震[1] 林代华[1] 刘维俊[1] 韩腾伟[1] 周淑姮[1] 邓艳琴[1,2] 

机构地区：[1]福建省疾病预防控制中心福建省人兽共患病研究重点实验室,福州350001 [2]福建医科大学,福州350004

出　　处：《中国人兽共患病学报》2017年第9期789-792,799,共5页Chinese Journal of Zoonoses

基　　金：福建省医学创新课题(2012-CXB-12);福建省自然科学基金项目(2017J01139)联合资助~~

摘　　要：目的了解福建省动物宿主感染巴贝虫状况。方法 2014-2016年本中心在福建各地捕鼠,笼日法捕鼠,采集鼠心脏血,同时采集牛、羊和狗的血液样本,采用PCR扩增巴贝虫18SrRNA基因,感染率的比较采用χ2检验或Fisher’s确切概率法。结果调查共布放鼠笼5 917笼次,捕鼠381只,鼠密度为6.44%。全省鼠类巴贝虫的感染率7.61%。家栖鼠中仅3只感染巴贝虫,感染率为1.68%。野鼠中26只感染巴贝虫,感染率为12.87%。野鼠感染率远高于家鼠,差异存在统计学意义(P<0.05)。地区分布看,闽中地区感染率最高,其次为闽东,闽南地区感染率最低,差异存在统计学意义(P<0.05)。其他动物,牛的血样未检出巴贝虫,狗和羊的血样中各1只检出巴贝虫,感染率分别为1.79%和0.55%。鼠类的巴贝虫感染率高于其他动物的感染率,差异存在统计学意义(P<0.05)。序列分析显示鼠类感染的均为田鼠巴贝虫。而犬中检出的巴贝虫为犬巴贝虫。结论福建省巴贝虫宿主动物中鼠类尤其是野鼠,感染率最高,可能是我省巴贝虫最重要的宿主。We explored the status of Babesia infection in natural hosts in Fujian Province. Rodents in Fujian Province were captured by the night trapping method during 2014-2016. Heart blood samples were collected. At the same time blood sam- ples of ox, goats and dogs were also collected, from which the fragments of 18S rRNA gene of Babesia were amplified by poly- merase chain reaction （PCR）. Data on infection rate were analyzed with Chi-square or Fisher exact test to indicate statistical significance. Results showed that 5 917 cages were laid and 381 rodents were captured, density of rodent was 6.44%. The o- verall Babesia infection rate in rodents was 7.61%, while infection rate in domesticated rodents was 1.68 %, and the infection rate in wide rodents was 12.87%. The infection rate was higher in wild rodents than that in domesticated rodents, and the sta- tistical analysis result revealed a significant difference. The infection rates in region of Central Fujian and Eastern Fujian was high, and no infection was found in Southern Fujian region. The statistical analysis result revealed a significant difference in infection rate among different regions. The infection rate of goats and dogs were determined to be 1.79 % and 0.55 %, and no infection was found in ox. The infection rate was higher in rodents than that in other host animals, and the statistical analysis result revealed a significant difference. It is suggested that the rodents, especially the wide rodents are the main natural hosts of Babesia.

关 键 词：巴贝虫 宿主动物 调查 福建 

分 类 号：R382[医药卫生—医学寄生虫学]