一测多评法用于香加皮提取物中7种C_(21)甾体皂苷成分的含量测定  

Simultaneous determination of seven C_(21) steroidal saponins in Cortex periplocae extract by quantitative analysis of multi-components by single-marker

作　　者：李瑞雪[1] 吴飞[1,2,3] 张继全 冯怡[1,2] 

机构地区：[1]上海中医药大学中药现代制剂技术教育部工程研究中心,上海201203 [2]上海张江中药现代制剂技术工程研究中心,上海201203 [3]上海中医药大学中药学院,上海201203

出　　处：《中国医药导报》2017年第27期21-24,共4页China Medical Herald

基　　金：上海市卫生和计划生育委员会进一步加快中医药事业发展三年行动计划项目(ZY3-CCCX-3-5001);上海市科学技术委员会科研计划项目(15DZ2292000)

摘　　要：目的以一测多评法建立香加皮提取物中7种C21甾体皂苷成分的高效液相色谱分析方法。方法采用Capcell Pak C_(18)色谱柱,流动相乙腈-0.2%甲酸水溶液梯度洗脱,检测波长263 nm,以杠柳苷A(PSA)为内参物,建立其与杠柳苷K(PSK)、杠柳苷R(WJ53)、杠柳苷E(WJ521)、杠柳苷D(PSD)、杠柳苷Q(WJ33)和杠柳苷O(WJ32)的相对校正因子,并进行含量计算,实现一测多评,并将一测多评法测定结果与外标法测定结果对比,验证一测多评法的准确性。结果 PSA与PSK、WJ53、WJ521、PSD、WJ33和WJ32的相对校正因子分别为0.867、0.867、0.847、0.994、0.922和1.029。采用校正因子计算的含量值与外标法实测值之间没有显著性差异。结论该方法准确可靠,简便可行,可作为香加皮提取物中间体多指标成分测定的质量控制方法。Objective T,, establish a high performance liquid chromatography method for quantitative analysis of seven C21 steroidal saonins in Cortex periplocae extract by quantitative analysis of multi-components by single-marker. Methods The high performance liquid chromatography analysis was performed on a Capcell Pak C18 column with the detection wavelength at 263 nm. The mobile phase was water containing 0.2% formic acid and acetonitrile in the gradient mode. Periploside C （PSA） was used as the internal marker to establish relative correction factors between PSA and periploside K （PSK）, periploside R （WJ53）, periploside E （WJ521）, periploside D （PSD）, periploside Q （WJ33）, periploside O （WJ32）, and the relative correction factors would be used in the calculation of their contents. The result of quantitative analysis of multi-components by single-marker was compared with external standard method, so as to verify the accuracy of quantitative analysis of multi-components by single-marker. Results The relative correction factors between PSA and PSK, WJ53, WJ521, PSD, WJ33, WJ32 was 0.867, 0.867, 0.847, 0.994, 0.922 and 1.029, respectively. The content values calculated by correction factors and measured values of external standard method did not show significant differences. Conclusion The established quantitative analysis of multi-components by single-marker method is feasible, accurate, simple and can be used as a method for the determination of multi-component indexes in the extract of Cortex periplocae.

关 键 词：香加皮提取物 C21甾体皂苷 一测多评法 相对校正因子 杠柳苷A 高效液相色谱法 外标法 

分 类 号：R284.1[医药卫生—中药学]