TSP1缺失可降低巨噬细胞的迁移和相关炎症表型  

Thrombospondin 1 deficiency reduces the migration of macrophages and related inflammatory phenotypes

作　　者：童晓鹏[1] 张敏[1] 

机构地区：[1]西藏民族大学高原相关疾病与基因相关研究实验室生命科学基础研究实验室,咸阳712082

出　　处：《现代免疫学》2017年第5期374-378,共5页Current Immunology

基　　金：国家自然科学基金(81560732;81560668);西藏自治区高校青年教师创新支持计划项目(QC2015-61);西藏民族大学重大培育项目(15MYZP02)

摘　　要：通过构建肥胖小鼠模型,探讨血小板反应蛋白1(thrombospondin 1,TSP1)在与肥胖相关的炎症反应中的作用及机制。用高脂食物喂养TSP1-/-的雄鼠和同源的WT雄鼠16周,收集血液并提取腹腔巨噬细胞,检测血浆MCP-1的水平;利用real-time PCR技术检测腹腔巨噬细胞内炎症因子IL-6、TNF-α、MCP-1和PAI-1的表达量;进一步采用Transwell技术分析巨噬细胞的迁移和黏附能力。得出以下结果:1.Real-time PCR结果显示,TSP1-/-小鼠巨噬细胞内IL-6、TNF-α、MCP-1和PAI-1mRNA表达量下降;2.Transwell结果显示,TSP1-/-小鼠的巨噬细胞向TSP1迁移的能力降低;3.WT和TSP1-/-小鼠血浆和脂肪组织中MCP-1表达均无明显差异。因此可认为,TSP1可以调节脂肪组织中巨噬细胞功能和炎症因子表达,其机制可能是通过减少炎症反应和降低巨噬细胞迁移能力实现的。We investigated the effect and mechanism of thrombospondin 1（TSP1）in obesity-associated inflammation by an obesity mouse model.Male TSP1-/-mice and wild type littermate controls were fed with a high-fat（HF）diet for 16 weeks.Then blood was collected and peritoneal macrophages were extracted and plasma MCP-1levels were detected.Real-time PCR was used to measure the expression levels of IL-6,TNF-α,MCP-1and PAI-1.The Transwell assay was used to analyze migration and adhesion ability of macrophages.The results were：1.Real-time PCR results showed that the mRNA expression levels of IL-6,TNF-α,MCP-1and PAI-1were decreased in macrophages in TSP1-/-mice.2.Transwell assay showed that the ability of macrophages to migrate toward purified TSP1 was significantly slowed.3.There was no difference in MCP-1level in either plasma or adipose tissues between WT and TSP1-/-mice.In conclusion,TSP1 can participate in the inflammatory response by regulating the expression of inflammatory cytokines and the ability of macrophage migration.

关 键 词：血小板反应蛋白1 巨噬细胞 炎症 迁移和黏附 

分 类 号：R392.3[医药卫生—免疫学]