LAIR-1对人急性髓系白血病细胞HEL增殖、凋亡和迁移的影响  

Effects of LAIR-1 on proliferation,apoptosis and migration of human acute myeloid leukemia cell line HEL

作　　者：吴肖婕 周嘉迪 曹奇志[1] 薛江楠[1] 

机构地区：[1]滨州医学院免疫学教研室,烟台264003

出　　处：《现代免疫学》2017年第5期385-389,共5页Current Immunology

基　　金：国家自然科学基金(81273200;81672757)

摘　　要：研究人白细胞相关免疫球蛋白样受体1(leukocyte-associated immunoglobulin-like receptor 1,LAIR-1)对人红白血病细胞HEL增殖、凋亡和迁移的影响,探讨LAIR-1在急性髓系白血病(acute myeloid leukemia,AML)中的作用。采用LAIR-1慢病毒表达载体(LV-LAIR-1)转染HEL细胞,建立稳定高表达LAIR-1的HEL细胞株,分别采用流式细胞术、激光扫描共聚焦显微技术、western blotting和RT-PCR鉴定LAIR-1分子的表达效率。采用CCK-8试剂盒、FITC Annexin V凋亡试剂盒和Transwell迁移实验分别检测LAIR-1对HEL细胞增殖、凋亡和迁移能力的影响。实验结果显示,经LVLAIR-1转染的HEL细胞高表达LAIR-1分子,阳性率达到85%以上;功能实验结果显示,与对照组细胞相比,LAIR-1能够显著抑制HEL细胞的增殖(P<0.001),并且促进其凋亡(P<0.01),但是对细胞迁移能力无明显影响(P>0.05)。上述研究结果显示,LAIR-1分子的表达能够影响人AML细胞的生物学功能。To determine the physiological function of leukocyte-associated immunoglobulin-like receptor 1（LAIR-1）in the pathogenesis of acute myeloid leukemia,we tested the effects of LAIR-1 on proliferation,apoptosis and migration in the erythroleukemia cell line HEL.Briefly,FCM,LSCM,western blotting and RT-PCR were used to confirm the lentivirusinduced over-expression of LAIR-1 in HEL cells.Subsequently,we used CCK-8kit,FITC Annexin V kit and Transwell experiment to test the effects of LAIR-1 on proliferation,apoptosis and migration in HEL cell lines.Our results demonstrated that lentivirus-mediated over-expression of LAIR-1 significantly suppresses the proliferation of HEL cells,while the apoptosis was enhanced.However,no difference in the migration was observed.Overall,these data indicated that the expression of LAIR-1 can affect some biological activities of acute myeloid leukemia cells.

关 键 词：LAIR-1 白血病 增殖 凋亡 迁移 

分 类 号：R392.11[医药卫生—免疫学]