TRAIL基因修饰的树突状细胞诱导黑色素瘤细胞凋亡的实验研究  

Improved anti-tumor activity of TRAIL-modified dendritic cell in melanoma cells

作　　者：张文颖[1] 袁海花[1] 姜斌[1] 王美玲[1] 龚玉芳[1] 刘峰[1] 

机构地区：[1]上海交通大学医学院附属第九人民医院,上海201999

出　　处：《现代免疫学》2017年第5期390-394,共5页Current Immunology

基　　金：上海市宝山区科委资助项目(13-E3)

摘　　要：为研究TRAIL基因修饰的树突状细胞诱导黑色素瘤细胞凋亡的作用,研究用携带TRAIL基因的重组腺病毒转染小鼠来源的树突状细胞(dendritic cell,DC),采用流式细胞术(flow cytometry,FCM)检测DC TRAIL蛋白的表达水平;将DC-TRAIL与B16黑色素瘤细胞混合培养,显微镜观察细胞生长情况,FCM检测B16细胞的凋亡情况;构建小鼠黑色素瘤模型,分别将DC-TRAIL、DC和PBS皮下注射于肿瘤接种部位,观察小鼠的瘤体生长情况,测量瘤体的体积。结果显示:用Ad-TRAIL转染DC后DC可高表达TRAIL。DC-TRAIL组与DC组相比可明显诱导肿瘤细胞凋亡。用DC-TRAIL、DC和PBS处理黑色素瘤移植小鼠2周后,发现DC-TRAIL组小鼠平均肿瘤体积为(0.33±0.10)cm^3,DC组小鼠平均肿瘤体积为(1.32±0.29)cm^3,PBS组小鼠平均肿瘤体积为(3.01±0.52)cm^3。与DC组和PBS组相比,DC-TRAIL组可明显抑制肿瘤生长。To evaluate the effects of TRAIL-modified dendritic cells on apoptosis of melanoma cells,we transferred dendritic cells with Ad-TRAIL.Expression of TRAIL could be detected by flow cytometry.DC-TRAIL was cultured with melanoma cells.The cell growth was observed under microscope and the apoptosis was detected by flow cytometry.The subcutaneous xenograft model of mouse melanoma cells was established and treated respectively with DC-TRAIL,DC and PBS.Tumor volume was measured.We found that Ad-TRAIL could effectively transfer DC.DC overexpressing TRAIL showed potent cytotoxicity against melanoma cells through the induction of apoptosis.The growth of xenograft tumor of melanoma cells in AdTRAIL group was significantly inhibited compared with that in DC and PBS groups.In conclusion,DC modified by Ad-TRAIL can inhibit proliferation of melanoma cells in vitro and vivo.It might be used for gene therapy of melanoma cells.

关 键 词：肿瘤坏死因子相关凋亡诱导配体 树突状细胞 黑色素瘤 

分 类 号：R392.12[医药卫生—免疫学]