猪带绦虫重组BCG-TSOL18疫苗诱导仔猪的保护性免疫应答  

Protective immune responses induced by a recombinant BCG-TSOL18 vaccine of Taenia soliumin piglets

作　　者：周必英[1] 杨凤娇[1] 刘美辰[1] 周泠[2] 贾启[1] 江楠[1] 王灵军[1] 

机构地区：[1]遵义医学院寄生虫学教研室,遵义563000 [2]遵义医学院附属医院中医肛肠科,遵义563000

出　　处：《现代免疫学》2017年第5期399-403,共5页Current Immunology

基　　金：贵州省科技厅社会发展攻关项目(黔科合SY字[2011]3038号)

摘　　要：研究猪带绦虫重组BCG-TSOL18疫苗免疫仔猪,猪带绦虫卵攻击后诱导其保护性免疫应答的情况。将12头40d龄健康仔猪均分为3组:分别为1011 CFU重组BCG-TSOL18疫苗灌胃组、BCG对照组和PBS对照组。每2周免疫1次,共免疫2次。于末次免疫4周后用猪带绦虫卵进行攻击感染,3月后剖杀仔猪,分离囊尾蚴,计算减蚴率;取前腔静脉血,分离血清和制备外周血单个核细胞(peripheral blood monouclear cell,PBMC)。采用ELISA法检测血清IgG、IgG1及IgG2a水平以及PBMC培养上清液中IL-2、IFN-γ、IL-4及IL-10的水平;MTT比色法检测PBMC增殖水平。重组BCG-TSOL18疫苗免疫组猪带绦虫卵攻击后诱导仔猪获得79.44%的保护力。与BCG和PBS对照组比较,重组BCG-TSOL18疫苗组血清IgG、IgG2a水平显著升高(t=13.026,P=0.000;t=13.253,P=0.000;t=7.284,P=0.000;t=8.453,P=0.000),IgG1水平显著降低(t=-9.118,P=0.000;t=-10.372,P=0.000);抗原刺激下PBMC显著增殖(t=14.929,P=0.000;t=14.665,P=0.000);PBMC培养上清液IL-2、IFN-γ水平显著升高(t=9.768,P=0.000;t=10.496,P=0.000;t=22.504,P=0.000;t=23.204,P=0.000),IL-4和IL-10水平显著降低(t=-18.659,P=0.000;t=-19.495,P=0.000;t=-26.258,P=0.000;t=-27.402,P=0.000)。猪带绦虫重组BCG-TSOL18疫苗可诱导仔猪产生Th1型的保护性免疫应答,从而抵抗猪带绦虫卵的感染攻击。To study protective immune responses induced by a recombinant BCG-TSOL18 vaccine of Taenia soliumin piglets,12 healthy piglets of 40 days old were randomly divided into three groups：1011 CFU rBCG-TSOL18 vaccine intragastric immunization group,BCG control group and PBS control group.A total of two times of immunization were conducted,once every two weeks.Piglets were challenged with T.soliumeggs on the 4th week after the last immunization and killed in 3months after infection.The cysticercus was separated to count and calculate the reduction rate.Blood from precaval vein was collected to separate serum and prepare peripheral blood lymphocytes（PBMC）.The levels of IgG,IgG1 and IgG2ain sera and the levels of IL-2,IFN-γ,IL-4and IL-10 in PBMC culture supernatant were determined by ELISA.The level of PBMC proliferation was tested using methyltetrazolium（MTT）assay.After immunization with rBCG vaccine of T.soliumand challenged with T.solium eggs,the acquired protection of piglets was 79.44%.Compared with BCG control group and PBS control group,the levels of IgG and IgG2 ain serum were significantly increased（t=13.026,P=0.000;t=13.253,P =0.000;t=7.284,P =0.000;t=8.453,P =0.000）,while the level of IgG1 was significantly decreased（t=-9.118,P =0.000;t=-10.372,P =0.000）.With the stimulation of recombinant TSOL18 antigen,the level of PBMC proliferation was significantly increased（t=14.929,P =0.000;t=14.665,P =0.000）.The levels of IL-2and IFN-γin PBMC culture supernatant were significantly increased（t=9.768,P =0.000;t=10.496,P =0.000;t=22.504,P =0.000;t=23.204,P =0.000）,while the levels of IL-4 and IL-10 was significantly decreased（t=-18.659,P =0.000;t=-19.495,P =0.000;t=-26.258,P =0.000;t=-27.402;P =0.000）.Therefore,the recombinant BCG-TSOL18 vaccine of Taenia solium might induce a protective Th1 type immune response,thus protecting piglets against the challenge of T.soliumeggs.

关 键 词：猪带绦虫 重组BCG-TSOL18疫苗 保护性免疫应答 仔猪 

分 类 号：R383.32[医药卫生—医学寄生虫学]