人类Grx3蛋白与转录因子p65相互作用的研究  

The Interaction Study Between Human Grx3 Protein and Transcription Factor p65

作　　者：胡可越 程宁辉 王新泉[1] HU Ke-yue CHENG Ning-hui WANG Xin-quan(School of Life Sciences, Tsinghua University, Beijing 100084, China)

机构地区：[1]清华大学生命科学学院,北京100084

出　　处：《中国生物工程杂志》2017年第9期1-6,共6页China Biotechnology

摘　　要：巯基氧化还原酶在生物体的系统内担当着非常重要的角色,其主要介导的是巯基和二硫键之间的相互转化的反应。人类Grx3蛋白作为一种巯基氧化还原酶在谷氧还蛋白系统中有着非常重要的作用。已有的研究表明,Grx3蛋白对NF-κB信号通路的活性有十分显著的影响。p65(RELA)是人类机体内十分重要的一种蛋白质,其在NF-κB信号通路有着重要的调控作用,几乎调控着NF-κB信号通路中的所有下游反应。通过大肠杆菌提纯表达的p65与Grx3在体外条件下进行偶联实验,为p65与Grx3存在相互作用提供了强有力的证据,同时也为Grx3蛋白对NF-κB信号通路的影响机制提供了一个可能的解释。Oxidation-reduction system exists in almost all organisms,and thiol-redox enzymes play a very important part in the oxidation-reduction system. Thiol-redox enzymes regulate the conversion between thiol group and disulfide bond. Thiol-redox enzymes exist in both glutaredoxins system and thioredoxins system in human body. Our target protein Grx3 is one of the most important role in glutaredoxins system. p65（ RELA） a very important protein in human body which has a very close relation with inflammatory reaction regulates the NF-κB signal pathway,it almost control all of the cascade reaction of NF-κB signal pathway,while other studies has shown that Grx3 has a significant influence on NF-κB signal pathway. There is a connection between p65 and Grx3 in vivo environment by colocalisationexperiment and p65 and Grx3 protein purify from E. coli can crosslink in a specific in vitro environment which provide a significant proof of interaction between Grx3 and p65 in vitro environment and explains how Grx3 get involved in the NF-κB signal pathway as while.

关 键 词：人类谷氧还蛋白Grx3 人类p65(RELA)蛋白 蛋白质偶联 

分 类 号：Q819[生物学—生物工程]