新型可控性栓塞剂MNPs-tTF的构建  

Construction of a Neotype Controllability Magnetic Targeting Restructuring Truncated Tissue Factor

作　　者：陈小丽 邹明园 谢鑫 王生育[1] 吴婷 苏金华 CHEN Xiao-li ZOU Ming-yuan XIE Xin WANG Sheng-yu WU Ting SU Jin-hua(Cancer Research Center, Medical College, Xiamen University, Xiamen 361005, China Laboratory Animal Center, Xiamen University, Xiamen 361005, China Department of Oncology, Chenggong Hospital Affiliated to Xiamen University, No. 174 Hospital of People＇s Liberation Army, Xiamen 361000, China)

机构地区：[1]厦门大学抗癌研究中心,厦门361005 [2]解放军第174医院厦门大学附属成功医院肿瘤科,厦门361000 [3]厦门大学实验动物中心,厦门361005

出　　处：《中国生物工程杂志》2017年第9期60-64,共5页China Biotechnology

基　　金：福建省科技计划重点项目(2014Y0082);福建省属公益类项目(2015R1036-3;2016R1034-2)资助项目

摘　　要：目的:构建新型的具有可控性且能诱发动物血栓的栓塞剂——磁靶向凝血蛋白,从而为动物血栓模型的建立提供新工具。方法:使用化学共沉淀法合成磁流体(magnetic nanoparticles,MNPs),通过透析对其纯化后用超导量子干涉磁强针(SQUID)测定其磁性能;利用基因工程方法表达截短组织因子(truncated tissue factor,tTF)(又称为凝血蛋白)并纯化与鉴定;采用戊二醛交联法构建磁靶向凝血蛋白(MNPs-tTF),体外分析其磁控靶向性及凝血效力。结果:成功构建了磁靶向凝血蛋白MNPs-tTF,体外实验证实其保留有磁流体MNPs的磁控靶向性,以及凝血蛋白tTF的凝血效力。结论:成功构建了磁靶向凝血蛋白MNPs-tTF,为血栓模型动物的建立提供一种新工具。Objective： To establish a neotype controllability magnetic targeting restructuring truncated tissue factor s for establish animal models of thrombosis. Methods： Magnetic nanoparticles（ MNPs） were prepared by chemical coprecipitation,and employed the superconducting quantum interference device to characterized the MNPs magnetization. The truncated tissue factor（ tTF） were expressed efficiently in E. coli and purification by gene engineering technology. MNPs conjugated the tTF by glutaraldehyde to compose MNPs-tTF. In vitro analysis the effects of magnetic targeting and blood coagulation of MNPs-tTF. Result： The magnetic targeting restructuring truncated tissue factor composites were build successfully. They are reserve efficient coagulation effect and magnetic targeted ability in vitro. Conclusion： We have successfully prepared magnetic targeting thrombin system MNPs-tTF have been successfully prepared,which maybe provide a basis for developing the establish animal models of thrombosis.

关 键 词：磁流体 截短组织因子 血栓 

分 类 号：Q819[生物学—生物工程]