干细胞3D支架的研究进展  

Advances in Stem Cell 3D Scaffolds

作　　者：徐竹[1] 诸葛启钏[1] 黄李洁[1] XU Zhu ZHUGE Qi-chuan HUANG Li-jie(Department of Neurosurgery, First Affiliated Hospital, Wenzhou Medical University, Wenzhou 325000, Chin)

机构地区：[1]温州医科大学附属第一医院,温州325000

出　　处：《中国生物工程杂志》2017年第9期112-117,共6页China Biotechnology

基　　金：温州市公益性科技计划项目(Y20150042);浙江省医药卫生重大科技计划(WKJ2013-2-022);浙江省医药卫生科技计划(2016RCA022)资助项目

摘　　要：干细胞是一类具有无限增殖潜能,可自我更新的细胞,不同的培养或诱导条件下能够分化成为不同的细胞类型。目前,随着医学治疗手段及干细胞研究的不断发展,发现干细胞可应用于很多疾病的治疗,干细胞临床应用日益广泛,逐渐成为科研工作者研究的热点。然而干细胞移植进入生物体后繁殖效率很低,无法达到需求的量,因此如何对干细胞在生物体外进行大量培养扩增、在生物体内如何更稳定地增殖分化成为迫切需要解决的难题。当前干细胞最主要的培养方法仍是2D培养,2D培养无法模拟体内的3D微环境,繁殖效率较低,正是由于2D培养局限性太多,促使国内外学者对3D培养技术和3D支架材料进行深入探索,并取得了大量成果。对3D培养技术在干细胞中的发展与应用进行概述和展望。Stem cells are characterized by the capacity of self-renewing and umlimited proliferation and differentiation. They can differentiate into specialized cells under different culture or induced conditions. At present,with the development of medical treatment and stem cell research,it is found that stem cells can be used for the treatment of many diseases. The clinical application of stem cells is in a vast need. However,stem cell transplantation efficiency is very low in vivo. It is still need to figure out the method of stem cells expansion,stable proliferation and differentiation. Currently,the main culture method of the stem cells is still 2D culture. 2D culture can not simulate the 3D microenvironment in the body,and the reproductive efficiency is low. There are emerging results to overcome the limition of 2D culture method by the experts. Here the development and application of 3D culture technologies of stem cells were reviewed.

关 键 词：干细胞 3D微环境 3D培养 支架 

分 类 号：Q813[生物学—生物工程]