胆结石小鼠肝脏核受体基因LRH-1及其调控基因SRBI的表达  

Gene expression of liver nuclear receptor LRH-1 and scavenger receptor class B type Ⅰ gene in cholesterol gallstone mice

作　　者：郭嵩 杨士勇[2] 曹红勇[2] 朱响[3] 任明 

机构地区：[1]南京中医药大学附属医院(江苏省中医院)普外科,江苏南京210000 [2]南京医科大学附属南京医院(南京市第一医院)普外科,江苏南京210006 [3]南京医科大学附属苏州医院苏州市立医院东院肝胆胰外科,江苏南京215000

出　　处：《中国现代普通外科进展》2017年第7期511-514,共4页Chinese Journal of Current Advances in General Surgery

摘　　要：目的:探讨胆固醇结石(GS)小鼠肝脏的核受体基因——肝核受体类似物1(LRH-1)及其调控基因BⅠ型清道夫受体(SRBI)的表达。方法:C57BL/6小鼠40只,均为雌性,10周龄,体重30~35 g。20只为一组,分别予以正常饮食(对照组)及高脂饮食(胆石组)喂养10周后处死。病理切片检查小鼠胆囊壁细胞组织学改变,实时定量PCR法测定肝脏LRH-I及SRBI的表达量。Western blot方法测定肝脏上述基因蛋白的表达量。结果:胆结石小鼠胆囊壁细胞呈现炎性改变。胆石组小鼠LRH-I mRNA及蛋白表达均高于对照组(P<0.01),SRBI mRNA及蛋白表达量较对照组升高(P<0.01)。结论:小鼠肝脏LRH-1基因的表达异常与胆囊胆固醇结石形成有关,同时SRBI的表达可能与结石形成有关,共同促进了小鼠胆结石的形成。Objective： To investigate the expressions of liver receptor homolog 1 （LRH-1） and scavenger receptor B type I （SRBI） gene in cholesterol gallstone （CGS） mice. Methods： Forty C57BL/6 mice, 20 with cholesterol gallstone （GS）and 20 controls without gallstones （GSF） were enrolled in this study, mRNA and protein expression of LRH-1 and SRBI genes were determined by RT-PCR and Western blot. Results： Gallbladder walls of GS mice were thicker with increased stromal granulocyte infiltration. The expression levels of LRH-1 genes were significantly higher in GS mice than in controls（P〈0.01）. The expression levels of SRBI genes were also significantly higher in GS mice than in controls（P〈0.01）. Conclusion： The increased expression of LRH-1 and SRBI gene may be related to GS disease.

关 键 词：胆结石 肝脏核受体类似物1 B1类清道夫受体 

分 类 号：R657.42[医药卫生—外科学]