基于电聚合技术的微生物膜制备方法  

Method of Microbial Biofilm Preparation Based on Electropolymerization Technology

作　　者：胡敬芳[1] 李玥琪 高国伟[1,2] 夏善红 

机构地区：[1]北京信息科技大学,传感器重点实验室,北京100101 [2]中国科学院电子学研究所,传感技术联合国家重点实验室,北京100080

出　　处：《仪表技术与传感器》2017年第9期13-16,29,共5页Instrument Technique and Sensor

基　　金：北京市自然科学基金资助项目(4154070);北京市优秀人才培养资助基金项目(2014000020124G104);传感技术联合国家重点实验室基金课题(SKT1307)

摘　　要：微生物膜的制备是影响BOD生物传感器性能的关键环节之一。基于电聚合技术,提出一种在电极表面制备微生物膜的新方法,研制成一种可用于快速检测水质生化需氧量(BOD)的电化学生物传感器。首先在金电极表面电聚合一层促进微生物代谢的电子媒介体——聚中性红膜(PNR/Au),然后通过电聚合掺杂有铜绿假单胞菌的吡咯溶液,在聚中性红膜表面制备一层铜绿假单胞菌-聚吡咯膜(PA-PPy),在电极表面形成一种具有双层结构的微生物膜(PA-PPy/PNR/Au),实现对水质BOD的快速检测。对微生物的固定条件和传感器的线性响应进行了研究,结果表明,这种固定方法对所固定的微生物的活性影响小,在BOD测定过程中,该传感器表现出良好的抗干扰性、重复性和稳定性,检测下限为3 mg/L。The microbial biofilm preparation is one key factor of influencing the performance of a biochemical oxygen demand（ BOD） biosensor.A new method based on electropolymerization technology for microbial biofilm preparation on electrode surface was proposed in this paper,and a fast BOD electrochemical biosensor was developed. The first step was electopolymerization of polyneutral red（ PNR） film,the artificial electronic mediator for promoting microbial respiration and metabolism.Then a layer of P.aeruginosa-polypyrrole（ PA-PPy） was modified on PNR film via electropolymerization of pyrrole doped with PA. modified electrode.The two layered microbial biofilm.The microorganism immobilization condition and the biosensor linear response were investigated for the microbial biofilm preparation PA-PPy/PNR/Au was formed on the electrode surface,implementing rapid test for water quality BOD.The results showed that the proposed immobilization method has little influence on the immobilized microorganism on electrode surface,and the developed biosensor has good anti-interference performance,repeatability and stability,with the low detection limit of 3 mg/L in the process of BOD testing.

关 键 词：微生物膜 电聚合 聚吡咯 电化学生物传感器 BOD快速检测 

分 类 号：TP212[自动化与计算机技术—检测技术与自动化装置]