2013—2016年四川省猪繁殖与呼吸综合征病继发细菌感染的病原学分析  

Etiology analysis of the bacterial secondary infection of PRRSV in Sichuan Province from 2013 to 2016

作　　者：李幽幽 李小璟 龚双燕[1] 敖英 朱玲[1,2] 徐志文[1,2] 

机构地区：[1]四川农业大学动物医学院,四川成都611134 [2]四川农业大学四川省动物疫病与人类健康重点实验室,四川成都611134

出　　处：《浙江农业学报》2017年第9期1437-1444,共8页Acta Agriculturae Zhejiangensis

基　　金：国家"十二五"科技支撑计划(2015BAD12B04-2.3);四川省科技支撑计划(2014NZ0043)

摘　　要：为给近几年临床控制和治疗PRRSV继发感染提供参考依据,对2013—2016年来自四川省各地感染PRRSV的112头病猪病料进行细菌分离,经形态学观察、选择培养、生化鉴定。分离得到葡萄球菌(MRS)、链球菌、多杀性巴氏杆菌(PM)、大肠埃希菌(Escherichia coli)、猪胸膜肺炎放线杆菌(App)。其中葡萄球菌的分离率最高(70%),其次为多杀性巴氏杆菌(50%),大肠埃希菌(40%),猪胸膜肺炎放线杆菌(40%),链球菌(30%);继发感染主要发生于肝脏和肺脏,其次是脾和心包积液。动物试验结果表明,多杀性巴氏杆菌、大肠埃希菌、链球菌、胸膜肺炎放线杆菌具有致病性。药敏试验结果表明:多杀性巴氏杆菌对环丙沙星敏感,对庆大霉素中度敏感;大肠埃希菌对丁胺卡那敏感,对氨苄西林、庆大霉素、新霉素中度敏感;链球菌对卡那霉素中度敏感;猪胸膜肺炎放线菌对环丙沙星中度敏感。This experiment was conducted to provide references for clinical management and treatment of PRRSV secondary infections. In this study,morphology observation,selective culture,biochemical identification were used to identify the secondary infective bacteria of PRRSV around 112 piglets from Sichuan province in 2013-2016.Staphylococcus aureus,Streptococcus,Pasteurella multocida,Escherichia coli and Actinobacillus pleuropeumoniae were isolated. Isolation rate of Staphylococcus aureus was the highest（ 70%）,followed by Pasteurella（ 50%）,Escherichia coli（ 40%）,Actinobacillus pleuropneumoniae（ 40%）,Streptococcus（ 30%）. Secondary infection occurred mainly in the liver and lungs,followed by spleen and pericardial effusion. Animal experiments showed that Pasteurella multocida,Escherichia coli,Streptococcus,Actinobacillus pleuropneumoniae were pathogenic. Susceptibility testing showed that Pasteurella multocida was highly sensitive to ciprofloxacin,moderately sensitive to gentamicin; Escherichia coli was highly sensitive to amikacin,and moderately sensitive to ampicillin,gentamicin and neomycin; Streptococcus wasmoderately sensitive to kanamycin; Actinobacillus pleuropneumoniae was moderately sensitive to ciprofloxacin.

关 键 词：猪繁殖与呼吸综合征 细菌 继发感染 病原学 

分 类 号：S855.1[农业科学—临床兽医学]