消毒液中的过氧乙酸应用增敏动力学光充法与阳离子表面活性剂协同检测的效果  

The Effect of Sensitizing Light Filling Dynamics Method and Cationic Surfactant Synergistic Peracetic Acid in the Detection of Disinfection Liquid

作　　者：马晓梅[1] 

机构地区：[1]菏泽市立医院供应室,山东菏泽274000

出　　处：《中外医疗》2017年第22期26-30,共5页China & Foreign Medical Treatment

摘　　要：目的探讨消毒液中的过氧乙酸应用增敏动力学光充法与阳离子表面活性剂协同检测的效果。方法 2015年1月—2016年12月于菏泽市立医院进行检测,过氧乙酸在稀硫酸介质中对Fe(II)-邻二氮杂菲体系的显色反应的阻抑作用十分显著,在510 nm波长处检测阻抑体系的吸光度值。结果于0.05~1.5μg/m L范围内吸光度和过氧乙酸的线性关系为递减式,检出限为0.02μg/m L;在400~600 nm的波长范围内,对各体系的吸收光谱分别检测,V_(CH3COOOH)=0.00 m L(非阻抑体系)在510 nm时出现最大吸收峰,峰数单一,峰值较高,峰形尖锐,摩尔吸光系数=2.20×10~4时,出现强吸收,阻抑体系在510 nm处仍维持吸收最大值,吸收光谱形状未出现改变,过氧乙酸加入后吸光度值呈阶梯式降低;在阳离子表面活性剂的作用下,吸光度整体出现不同程度的增加,如十四烷基溴吡啶TPB及十六氨基溴吡啶CPB,十八烷基三甲基溴化铵STAB,相对来说,TPB-STAB混合体系的优越性十分显著,加入相同含量的STAB及TPB,吸光度值急骤上升,在最大吸收波长处摩尔吸光系数为2.20×10~4。在最初反应时间吸光度与反应时间呈正相关,但其达到最大吸光度值及趋于恒定的时间具有一定的差异;当P_(CH3COOOH)=0μg/m L时,非阻抑体系A值为5~8 min增大缓慢,9 min后趋于恒定,A值在1 h左右变化较小;当P_(CH3COOOH)=1.0μg/m L时,在1~5 min内阻抑体系A值上升迅速,6 min后较为稳定,在1 h左右A值的变化较小。结论微量过氧乙酸应用过氧乙酸对铁(II)-邻二氮杂菲分光光度法检测效果确切,可用于检测消毒液中的过氧乙酸。Objective This paper tries to study the effect of the application of the sensitization kinetics and the cationic surfactant on the peracetic acid in disinfectant solution. Methods The test was carried out in this hospital from January2015 to December 2016. The inhibitory effect of peracetic acid on the color reaction of Fe（II）-o-phenanthroline system in dilute sulfuric acid medium was significant, and the absorbance value of the inhibition system was detected at the wavelength of 510 nm. Results In the range of 0.05~1.5 μg/m L, the linear relationship between absorbance and peracetic acid was decreased, and the detection limit was 0.02 μg/m L; In the wavelength range of 400~600 nm, the absorption spectra of each system were detected in V_（CH3COOOH）=0.00 m L（non inhibitory system） appears in the 510 nm when the maximum absorption was in the peak, with single, high and sharp peak, molar absorption coefficient =2.20 ×10~4, with strong absorption, inhibitory system remained absorption maxima at 510 nm, the shape of absorption spectrum had changed, the absorbance value after adding peracetic acid was stepped lower; under influence of the active agent in the cationic surfactant, the overall light absorbance increased in different degree, such as fourteen TPB and sixteen alkyl bromide pyridine amino brominepyridine CPB, eighteen alkyl three methyl bromide STAB, relatively speaking, the superiority of the TPB-STAB hybrid system was very significant, adding the same amount of STAB and TPB, the absorbance value was rising rapidly. The molar absorptivity was 2.20×10~4 at the maximum absorption wavelength; There was a positive correlation between the absorbance of the initial reaction time and the reaction time, but the difference was significant when the maximum absorbance value and the time constant arrived; When P_（CH3COOOH）=0 μg/m L, non inhibitory system A=5~8 min increased slowly, it tended to be constant after 9 min; A value changed a little in about 1 hours; When P_（CH3COOOH）=1.0 ug/m L, the

关 键 词：动力学光度法 Fe(II)-邻二氮杂菲体系 过氧乙酸 阳离子表面活性剂 

分 类 号：R5[医药卫生—内科学]