铜绿假单胞菌负载Fe(Ⅲ)对水中六价铬的生物吸附性能研究  

Biosorption of hexavalent chromium on Pseudomonas aeruginosa pretreated with Fe(Ⅲ)

作　　者：喻浪风 吴平霄 陈梅青[1] 亢春喜 刘帅 朱能武 黄柱坚 YU Langfeng WU Pingxiao CHEN Meiqing KANG Chunxi LIU Shuai ZHU Nerlgwu HUANG Zhujian(School of Environment and Energy, South China University of Technology, Guangzhou 510006 The Key Lab of Pollution Control and Ecosystem Restoration in Industry Clusters, Ministry of Education, South China University of Technology, Guangzhou 510006 Guangdong Provincial Engineering and Technology Research Center for Environmental Risk Prevention and Emergency Disposal, South China University of Technology, Guangzhou 510006 4.Guangdong Engineering and Technology Research Center for Environmental Nanomaterials, Guangzhou 510006 College of Natural Resources and Environment,South China Agricultural University, Guangzhou 510006)

机构地区：[1]华南理工大学环境与能源学院,广州510006 [2]工业聚集区污染控制与生态修复教育部重点实验室,广州510006 [3]广东省环境风险防控与应急处置工程技术研究中心,广州510006 [4]广东省环境纳米材料工程技术研究中心,广州510006 [5]华南农业大学资源环境学院,广州510006

出　　处：《环境科学学报》2017年第10期3702-3712,共11页Acta Scientiae Circumstantiae

基　　金：国家重点研发计划(No.2017YFD0801000);国家自然科学基金(No.41673092;41472038;41273122);广东省科技计划项目(No.2014A020216002;2016B020242004);广州市科技计划项目(No.201604020064);广东特支计划百千万工程领导人才项目(No.201626011)~~

摘　　要：采用原位沉积技术将Fe(Ⅲ)负载于铜绿假单胞菌(Pseudomonas aeruginosa,简称Pa)表面制备了Fe(Ⅲ)与细菌的复合体(Fe-Pa),研究了Fe-Pa对水溶液中Cr(Ⅵ)的吸附特性,探讨了最佳合成条件、Fe-Pa投加量、溶液pH值、时间和Cr(Ⅵ)初始浓度等因素对Cr(Ⅵ)吸附效果的影响,同时利用SEM、FT-IR、XPS和Zeta电位对Fe-Pa进行表征分析.吸附实验结果显示,Fe(Ⅲ)浓度为600 mg·L^(-1)、细菌投加量为0.5 g·L^(-1)制备的Fe-Pa效果最佳;Fe-Pa去除Cr(Ⅵ)适宜于酸性条件进行;Fe-Pa对Cr(Ⅵ)的吸附速率较快,60 min内可达到吸附平衡,为自发的吸热吸附,且符合准二级动力学和Langmuir等温模型.表征结果表明,Fe(Ⅲ)成功地负载到铜绿假单胞菌上,为吸附Cr(Ⅵ)提供更多的活性位点,主要机制为静电吸附作用、络合作用和还原作用.经过4次吸附/再生后,Fe-Pa对Cr(Ⅵ)的吸附能力仍在72%以上,表明Fe-Pa具有较好的重复使用性.Pseudomonas aeruginosa was pretreated with Fe（ Ⅲ） solution to form a biosorbent named as Fe-Pa. The adsorption behavior characteristics of Cr（ Ⅵ） onto Fe-Pa in aqueous solution were investigated by evaluating optimum synthesis conditions,the efffects of biomass dosage,pH value,contact time and initial concentration of Cr（ Ⅵ）. The physico-chemical properties of Fe-Pa were performed by using Scanning electronic microscopy（ SEM）,Fourier transform infrared spectroscopy（ FTIR）,X-ray photoelectron spectroscopy（ XPS） and Zeta potential. The best preparation condition of Fe-Pa was under Fe（ Ⅲ） concentration of 600 mg·L^-1with P. aeruginosa dosage of 0.5 g·L^-1. The lower pH was more benefit for Cr（ Ⅵ） adsorption onto Fe-Pa.Cr（ Ⅵ） adsorption was a endothermic and spontaneous process and could reach equilibrium within 60 min. Pseudo-second-order kinetics model and Langmuir adsorption model fitted the experimental data well. Fe（ Ⅲ） was successfully loaded onto the surface of Pseudomonas aeruginosa,which provided a large number of activated sites for the adsorption of Cr（ Ⅵ）. Electrostatic interactions and surface complexation were inferred to be the main mechanisms of adsorption of Cr（ Ⅵ） on Fe-Pa. After 4 times of adsorption/regeneration test,the maximum removal efficiency of Cr（ Ⅵ） could maintain 72%,which indicates that Fe-Pa had an excellent reusability.

关 键 词：铜绿假单胞菌 Fe(Ⅲ) 生物吸附 Cr(Ⅵ) 

分 类 号：X703[环境科学与工程—环境工程]