UHPLC-MS法鉴定大鼠灌服开心散后血中远志糖酯类化合物及其代谢产物  

Identification of polygala oligosaccharide esters and their metabolites in rat plasma after oral administration of ethanol extract of Kai Xin San by UHPLC-MS

作　　者：汪娜[1] Hassan AHMAD 贾永明[1] 薛迪[1] Ram CHAULAGAIN 黄树明[2] 刘学伟[1] WANG Na Hassan AHMAD JIA Yong-ming XUE Di Ram CHAULAGAIN HUANG Shu-ming LIU Xue-wei(Qiqihar Medical University, Qiqihar 161006, China Heilongjiang University of Chinese Medicine, Harbin 150040, China)

机构地区：[1]齐齐哈尔医学院,黑龙江齐齐哈尔161006 [2]黑龙江中医药大学,黑龙江哈尔滨150040

出　　处：《药学学报》2017年第10期1592-1598,共7页Acta Pharmaceutica Sinica

基　　金：国家自然科学基金青年基金资助项目(81303248);黑龙江省普通本科高等学校青年创新人才培养计划(UNPYSCT-2016116);齐齐哈尔医学院校内博士创新基金资助项目(QY2016B-09);黑龙江省自然科学基金资助项目(H2015028);黑龙江省教育厅基本科研业务费基础研究项目(2016-KYYWF-0865)

摘　　要：本研究利用超高效液相色谱-飞行时间质谱联用技术(UHPLC-TOF/MS),借助液质联用工作站Masslynx V4.1分析软件,结合现有文献及相关数据库,鉴定了开心散60%乙醇提取物(KXS-60%E)中20个远志糖酯类成分;在此基础上,进一步指认并鉴定了灌服KXS-60%E后大鼠血中的14个远志糖酯类化合物及4个来源于远志糖酯类成分的代谢产物。以上研究结果表明,远志糖酯类化合物及其代谢产物是KXS-60%E的主要血中移行成分,原形及代谢产物可能是开心散防治AD的潜在药效物质基础。Using a UHPLC-TOF/MS method combined with software of Masslynx V4.1 and database in the literatures, a total of 20 polygala oligosaccharide esters（POEs） were identified in 60% ethanol extract of Kai Xin San（KXS-60%E）. Furthermore, 14 POEs as well as 4 POE-metabolites were identified in rat plasma. The results revealed that POEs and POE-metabolites which were identified in rat plasma, were key components in KXS-60%E and the potential bioactive compounds for KXS action in the treatment of AD.

关 键 词：开心散 血中移行成分 远志糖酯 代谢产物 

分 类 号：R917[医药卫生—药物分析学]