基于SCoT标记的陕产茜草野生居群遗传多样性分析  

The genetic diversity of wild populations of Rubia cordifolia L. in Shaanxi province based on SCoT analysis

作　　者：彭亮[1,2] 黄涛 张岗 胡本祥[1] 王媛媛 任瀛[1] 郭琦[1] 杨冰月[1,2] PENG Liang HUANG Tao ZHANG Gang HU Ben-xiang WANG Yuan-yuan REN Ying GUO Qi YANG Bing-yue(College of Pharmacy, Shaanxi University of Chinese Medicine, Xi ＇an 712046, China Shaanxi Collaborative Innovation Center of Chinese Medicinal Resources Industrialization, Xi ＇an 712046, China)

机构地区：[1]陕西中医药大学药学院,陕西西安712046 [2]陕西省中药资源产业化协同创新中心,陕西西安712046

出　　处：《药学学报》2017年第10期1621-1628,共8页Acta Pharmaceutica Sinica

基　　金：基金项目:陕产道地药材茜草野生变家种栽培技术规范化及质量评价研究(16JK1212);干旱胁迫及营养因子对茜草生长发育和品质的影响(15JK1210);陕产茜草的良种选育及其遗传多样性研究(11JS038)

摘　　要：茜草是我国传统中药材,近年来,其药材价格不断上涨,导致野生资源急剧减少,因此对其野生资源的遗传多样性进行评价,对茜草的资源保护、优良品种选育和开发利用意义重大。本文采用SCoT分子标记方法对陕西境内8个野生茜草居群64份种质进行了遗传多样性和遗传结构分析。结果表明,陕产野生茜草遗传多样性丰富,14条引物共扩增得到182条带,其中多态性条带163条,多态性百分比为89.56%;种群水平上Nei’s基因多样性指数(H)为0.293 6、Shannon指数(I)平均值为0.444 6、居群间遗传分化系数(Gst)为0.555 3、种群间基因流(Nm)为0.440 8;不同野生居群按遗传多样性水平排序为:安康>榆林>商洛>宝鸡>铜川>延安>渭南>咸阳。Mantel分析表明,陕产茜草野生居群间的亲缘关系与它们之间的地理距离存在显著的正相关性(r=0.776 4,P<0.05)。本文为陕产茜草野生居群的保护和资源利用提供了理论依据。Rubia cordifolia L. has been used as a traditional Chinese medicine for several centuries. In recent years, the resources of wild Rubia cordifolia have been declined sharply due to increased utilization and rising price. Therefore, it is of great urgency to evaluate and protect resources of wild plant of Rubia cordifolia. In our study, sixty-four individuals that represent eight wild populations of Rubia cordifolia L. were analyzed by Start Codon Targeted Polymorphism（SCoT） molecular markers. Genetic distance was calculated by POPGENE 3.2 software, cluster analysis was generated by NTSYS 2.10 software based on UPGMA method and Mantel Test was used to analysis the relationship between the genetic distances and geographical distance among the wild populations. The results showed a high genetic diversity of wild populations of Rubia cordifolia L. in Shaanxi province. A total of 182 bands were produced by 14 primers, among which 163 bands were polymorphic bands, and the percentage of polymorphic bands was 89.56%. The average value of Nei＇s genetic diversity index（H） was 0.293 6, Shannon＇s information index（I） was 0.444 6, genetic differentiation coefficient（Gst） was 0.555 3, and the gene flow（Nm） was 0.440 8, the wild populations were ranked by genetic diversity： AK YL SL BJ TC YA WN XY. Mantel Test analysis demonstrated that the significant correlation was found between the genetic distances and geographical distances（r = 0.776 4, P〈0.05）. There is a significant correlation between geographical and genetic distances. This study provides a theoretical basis for the protection and development of the resources of wild Rubia cordifolia L. germplasms.

关 键 词：茜草 SCoT 种质资源 遗传多样性 

分 类 号：R931[医药卫生—生药学]