pLXSN-Tum-5致人脐静脉内皮细胞凋亡作用及机制  

Growth inhibition and apoptosis induction effects of pLXSN-Tum-5 in human umbilical vein endothelial cells

作　　者：唐泽波 温娜 齐玲 历春[2] 盖晓东[2] 

机构地区：[1]吉林医药学院基础医学院,吉林吉林132013 [2]北华大学基础医学院

出　　处：《中国公共卫生》2017年第9期1360-1363,共4页Chinese Journal of Public Health

基　　金：吉林省科技厅项目(20130206050YY;20150101128JC)

摘　　要：目的探讨pLXSN-Tum-5病毒颗粒对人脐静脉内皮细胞(HUVEC)的生长抑制和诱导凋亡作用及机制。方法采用不同浓度的pLXSN-Tum-5病毒颗粒作用HUVEC,利用噻唑蓝法和Hoechst-33342染色法检测细胞生长及其形态变化;RT-PCR和Western blotting方法检测细胞中Bax、Bcl-2、caspase-3 mRNA和蛋白表达变化。结果与对照组(pLXSN)比较,pLXSN-Tum-5病毒颗粒转染组HUVEC的存活率明显降低,呈剂量效应关系;细胞内可见凋亡小体;对照组HUVEC内Bcl-2和caspase-3 mRNA和蛋白表达分别为(0.721±0.041)、(0.654±0.034)和(0.956±0.032)、(0.356±0.054);与对照组比较,20μmol/L pLXSN-Tum-5病毒颗粒转染组HUVEC内Bcl-2和caspase-3mRNA和蛋白水平[分别为(1.134±0.0524)、(1.012±0.0641)和(1.612±0.067)、(0.712±0.0647)]明显上调(P<0.05)。结论 Tum-5可抑制HUVEC增殖,诱导HUVEC凋亡,其机制可能与上调Bax/Bcl-2和caspase-3表达有关。Objective To explore growth inhibition and apoptosis induction effects of pLXSN-Tum-5 in human umbilical vein endothelial cells （HUVEC） and the mechanism of the effects.Methods HUVEC were treated with pLXSN-Tum-5 virus particles at different concentrations.Hoechst-33342 staining and 3-（4,5）-dimethylthiahiazo （-z-y1）-3,5-di-phenytetrazoliumromide （MTT） assay were utilized to monitor the growth states and morphological changes of HUVEC.Changes in the expressions of intracellular B cell lymphoma 2 （Bcl-2）,Bcl-2-associated X （Bax）,and caspase-3 at mRNA and protein levels were determined with real-time reverse transcription PCR （RT-PCR） and Western blot.Results The survival rate of HUVEC transfected with pLXSN-Tum-5 virus particles was significantly decreased in a dose-effect manner compared to that of the control （with pLXSN）.Apoptotic bodies were observed in the HUVEC transfected with pLXSN-Tum-5.Significantly upregulated mRNA and protein expressions of Bcl-2 （1.134±0.0524 vs.0.721±0.041 and 1.012±0.0641 vs.0.654±0.034） and caspase-3 （1.612±0.067 vs.0.956±0.032 and 0.712±0.0647 vs.0.356±0.054） were observed in the HUVEC transfected with 20 mol/L pLXSN-Tum-5 virus particles compared to those in the control HUVEC （P〈0.05 for all）.Conclusion Human Tum-5 could inhibit proliferation and induce apoptosis in HUVEC,and the mechanism of the effects may be related to upregulated expressions of Bax/Bcl-2 and caspase-3 in the HUVEC.

关 键 词：肿瘤抑素(tumstatin) Tum-5 细胞凋亡 人脐静脉内皮细胞(HUVEC) 抗血管生成 

分 类 号：R994.2[医药卫生—毒理学]