饮用水中亚硝胺类物质气相色谱质谱法检测应用  

Application of gas chromatography-mass spectrometry in detection of nitrosamines in drinking water

作　　者：冯媛美 李仪楠 刘冉[1] 浦跃朴[1] 尹立红[1] 

机构地区：[1]环境医学工程教育部重点实验室东南大学公共卫生学院,江苏南京210009

出　　处：《中国公共卫生》2017年第9期1400-1404,共5页Chinese Journal of Public Health

基　　金：国家自然科学基金(81573191)

摘　　要：目的建立优化固相萃取-气相色谱质谱(SPE-GC-MS)同时检测饮用水中9种亚硝胺类物质方法条件。方法通过固相萃取前处理以及优化后的气相色谱质谱法对样品净化浓缩,同时通过样品回收率、精密度、方法检出限(MDL)、测定下限、标准曲线对建立的方法进行验证。结果 9种亚硝胺的保留时间在9.819~20.541min,在10~1000μg/L均线性范围良好(R^2>0.999),MDL在2.27~6.20ng/L,低浓度(50ng/L)加标回收率为62.32%~89.71%,中(100 ng/L)、高浓度(200 ng/L)加标回收率为73.43%~109.66%,相对标准偏差(RSD)均<10,自来水加标回收率为74.62%~102.74%。结论建立的SPE-GC-MS法满足检测饮用水中9种亚硝胺类物质的要求。Objective To establish and optimize a solid phase extraction-gas chromatography-mass spectrometry（SPE-GC-MS）method for simultaneous detection of nine N-nitrosamines in drinking water.Methods The drinking water samples were purified and concentrated with SPE-GC-MS.Recovery,accuracy,method detection limit,lower limit,and standard curve were used to verify the established method.Results The retention time of nine N-nitrosamines was between 9.819 and 20.541 minutes.A good linearity within the concerntration from 10 μg/L to 1 000 μg/L was observed （R2〉0.999）.The detection limit of the method was between 2.27 ng/L and 6.20 ng/L.The recovery for low （50 ng/L） and medium （100 ng/L） to high concentration （200ng/L） were between 62.32%-89.71% and 73.43%-109.66%.The relative standard deviation （RSD） for all the detections were less than 10.The recoveries for all drinking water samples were between 74.62% and 102.74%.Conclusion The solid phase extraction with gas chromatography-mass spectrometry method established could meet the requirement for the detection of nine N-nitrosamines in drinking water samples.

关 键 词：亚硝胺 固相萃取-气相色谱质谱(SPE-GC-MS) 饮用水 

分 类 号：R123.1[医药卫生—环境卫生学]