新型抗真菌活性物质农抗N2粗提物对水稻种子萌发的影响  

Effects of Novel Antifungalmycin N2 Crude Extract from Streptomyces sp. N2 on Germination of Rice Seed

作　　者：吴志明[1,2] 钟敏 鹿承建[1] 朱曼宁 杜起风 李昆太 WU Zhi-ming ZHONG Min LU Cheng-jian ZHU Man-ning DU Qi-feng LI Kun-tai(College of Biological Sciences and Engineering, Jiangxi Agriculture University, Nanchang 330045 Jiangxi Engineering Laboratory for the Development and Utilization of Agricultural Mierobial Resources, Nanchang 330045)

机构地区：[1]江西农业大学生物科学与工程学院,南昌330045 [2]江西省农业微生物资源开发与利用工程实验室,南昌330045

出　　处：《生物技术通报》2017年第9期153-159,共7页Biotechnology Bulletin

基　　金：国家自然科学基金项目(31760546);江西省研究生创新专项资金资助项目(YC2016-S198);江西农业大学大学生创新创业计划项目(201610410022);江西省青年科学家培养对象计划项目(20142BCB23025);江西省教育厅科技项目(GJJ160389)

摘　　要：为探究不同浓度新型广谱农抗N2粗提物对水稻种子萌发的影响及作用机制,采用紫外-可见分光光度法考察了农抗N2粗提物浸种对水稻种子多项萌发指标、淀粉酶活力、可溶性总糖、可溶性蛋白及丙二醛含量的影响。结果表明:农抗N2粗提物对水稻种子萌发的作用主要表现为低浓度促进,高浓度抑制。0.23-1.15μg/m L农抗N2粗提物浸种均能有效增强种子活力,促进胚芽和胚根的生长,尤以0.58μg/m L处理组效果最佳;此外,淀粉酶总活力、可溶性总糖和蛋白质含量相较CK分别提高了28.27%、7.73%与6.49%,且差异显著;同时,相比CK处理组,农抗N2粗提物浸种能显著降低种子中丙二醛的含量。因此,适宜浓度农抗N2粗提物浸种有利于促进水稻种子萌发,提高物质代谢能力,增强水稻种子的渗透调节与组织保护能力。To investigate the effects of a novel antifungalmycin N2（AN2）crude extract from Streptomyces sp. N2 on germination of riceseed,the multi-index of germination,amylase activity,soluble sugar contents,soluble protein contents and the contents of malondialdehyde（MDA）in rice seeds were measured using ultraviolet-visible spectrophotometry. The results showed that the influence of AN2 on each indexwas correlated with the treated AN2 concentration. It was found that AN2 at low concentration promoted rice seed germination,while it at highconcentrations had an inhibitory effect. When treated with 0.23-1.15 μg/m L of AN2,the characteristics of seed germination was effectivelyimproved,and the growth of seedlings was significantly promoted,especially under the treatment with the concentration of 0.58 μg/m L. Inaddition,the content of soluble sugar,soluble proteins and amylase activity of optimal concentration treated groups was increased significantlythan control group by 28.27%,7.73% and 6.49%,respectively. In the 0.23-1.15 μg/m L of AN2 treatment group,the MDA content decreasedsignificantly. Therefore,proper concentration treatments promote germination capacity,improve enhance material metabolism and enhanceability of osmotic regulation.

关 键 词：链霉菌N2 水稻 种子萌发 生理特性 

分 类 号：S511[农业科学—作物学]