侧孢短芽孢杆菌A60的筛选及其对辣椒疫霉的室内防效测定  

Isolation of Brevibacillus laterosporus A60 and Its Greenhouse Control Efficiency Against Phytophthora capsica

作　　者：郝楠 仝赞华[1] 邱德文[1] HAO Nan TONG Zan-Hua QIU De-Wen(The Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100081)

机构地区：[1]中国农业科学院植物保护研究所,北京100081

出　　处：《生物技术通报》2017年第9期160-165,共6页Biotechnology Bulletin

摘　　要：采用平板对峙法对分离自海南43份土样的700余株细菌进行筛选,获得一株对辣椒疫霉病菌(Phytophthora capsici)具有高效抑制活性的拮抗菌A60,根据形态特征、生理生化特征和16S rDNA序列分析鉴定其为侧孢短芽孢杆菌(Brevibacillus laterosporus)。分别在辣椒和甜椒上进行室内防效测定,结果表明,加入拮抗菌A60的辣椒,在第3天时未发病,对辣椒疫霉病的防效达100%,第5天时为95.71%,第7天仍可达到92.22%;加入拮抗菌A60的甜椒,在第3天时未发病,防效达100%,第5天时为75.26%,第7天仍达73.20%,说明拮抗菌A60能够明显的降低发病率和病情指数,具有较好的防病持效性。A60 strain with high inhibition rate against Phytophthora capsici was screened from 700 strains isolated from 43 soil samplesin Hainan province. The A60 strain was identified as Brevibacillus laterosporus based on physiological and biochemical characteristics and a16 S r DNA gene sequence analysis. The control effects of A60 strain against Phytophthora on Capsicum annuum and Capsicum fructescens weredetermined in greenhouse. The results showed that the biocontrol efficacy of A60 strain on C. annuum L. were 100%,95.71% and 92.22%at 3rd,5th and 7th day,respectively. The control efficacy on C. fructescens were 100%,75.26% and 73.20% at 3rd,5th and 7th day,respectively. Our results highlight that the application of B. laterosporus A60 significantly reduce morbidity and disease index,i.e.,controlefficacy is solid.

关 键 词：辣椒疫霉 侧孢短芽孢杆菌 生物防治 

分 类 号：S436.418[农业科学—农业昆虫与害虫防治] S476.1[农业科学—植物保护]