亚砷酸钠中毒对大鼠海马组织神经元及肾功能的影响研究  

Impact of NaAsO_2 Poisoning on Rat Hippocampal Neurons and the Kidney Function

作　　者：秦文娟[1] 管东方[1] 邢国强[2] 史红娟 吕海龙[2] 姜玉峰[1] 

机构地区：[1]石河子大学医学院人体解剖与组织胚胎学教研室,新疆石河子市832002 [2]石河子大学第一附属医院肝胆外科,新疆石河子市832002 [3]衢州职业技术学院医学院,浙江省衢州市324000

出　　处：《中国全科医学》2017年第26期3246-3250,共5页Chinese General Practice

基　　金：国家自然科学基金资助项目(81360410)

摘　　要：目的观察亚砷酸钠(NaAsO_2)中毒对成年SD大鼠海马组织CA3区的神经元及肾脏形态学的影响。方法于2016年2—6月,48只12周龄雄性SPF级SD大鼠随机分为对照组及低、中、高染毒组,每组各12只。将NaAsO_2用蒸馏水分别配制成5、10、20 mg/kg溶液分别供低、中、高染毒组大鼠自由饮用,对照组可自由饮用蒸馏水。建造染砷模型12周后,取4组大鼠脑组织海马部分和肾组织,HE染色光镜观察海马组织神经元与肾脏形态学改变;同时测定海马组织与肾组织中超氧化物歧化酶(SOD)活力、还原型谷胱甘肽(GSH)及丙二醛(MDA)含量。结果不同剂量染毒组海马组织神经元与对照组比较,神经元胞体形态欠规则,排列稀疏且数量减少,镜下可见大量脱落及坏死的神经元。不同剂量染毒组的肾组织较对照组出现病理性改变,例如变性、坏死、组织出现水肿及炎性细胞浸润等。4组大鼠海马组织及肾组织SOD活力、GSH和MDA含量比较,差异均有统计学意义(P<0.05);其中低、中、高染毒组海马组织及肾组织SOD活力、GSH含量均低于对照组,MDA含量均高于对照组;中、高染毒组海马组织及肾组织SOD活力、GSH含量均低于低染毒组,MDA含量均高于低染毒组;高染毒组海马组织及肾组织SOD活力、GSH含量均低于中染毒组,MDA含量均高于中染毒组(P<0.05)。结论 NaAsO_2中毒可使大鼠海马组织神经元形态结构及肾组织结构损伤,同时提示氧化应激可能参与NaAsO_2致大鼠海马组织及肾组织的毒性作用。Objective To observe the changes in histology and morphology of hippocampal CA3 region neurons and renal tissues in adult SD rats after NaAsO2 poisoning. Methods This study was conducted from February to June in 2016. Forty-eight male SD rats（ SPF grade） aged 12 weeks were randomly assigned to the normal control group,low-,medium-and high-dose NaAsO2 infected groups with 12 in each,respectively treated by freely drinking of distilled water,5,10,20 mg/kg NaAsO2 solution for an intervention of 12 weeks. All the rats were sacrificed and the hippocampal and kidney tissues were taken at the end of intervention. Light microscope was used to observe the morphology changes in hippocampal and renal tissues stained by HE. The activity of superoxide dismutase（ SOD） and concentrations of reduced glutathione（ GSH） and malondialdehyde（ MDA） in these tissues were determined. Results The observation showed that,compared with the control group,in hippocampal region of the NaAsO2 infected groups, the nerve cells were irregular with decreased number and enlarged interspaces,moreover,a large number of exfoliated and necrotic nerve cells could be seen; pathological changes were found in the renal tissues, including degeneration, necrosis, edema and inflammatory cell infiltration. The activity of SOD and concentrations of GSH and MDA in hippocampal and renal tissues differed significantly among the four groups（ P〈0. 05）,specifically,the NaAsO2 infected groups had lower activity of SOD and concentration of GSH but higher concentration of MDA in hippocampal and renal tissues than the control group did; the medium-and high-dose NaAsO2 infected groups had lower activity of SOD and concentration of GSH but higher concentration of MDA in hippocampal and renal tissues than the low-dose NaAsO2 infected group did; the high-dose NaAsO2 infected group had lower activity of SOD and concentration of GSH but higher concentration of MDA in hippocampal and renal tissues than the medium-dose NaAsO2 infected group did（ P〈0. 05

关 键 词：砷中毒 CA3区 海马 肾功能 氧化应激 

分 类 号：R595.2[医药卫生—内科学]