煅烧异种骨的生物相容性研究  

Biocompatibility research of true bone ceramics

作　　者：乔玮 任晓琦 石浩 李晶[1] 杨婷[1] 马绍英[1] 赵亚平[1] 苏成忠[1] 李宝兴[1] 

机构地区：[1]中国辐射防护研究院山西省组织库山西奥瑞生物材料有限公司,太原030006

出　　处：《中国修复重建外科杂志》2017年第10期1250-1255,共6页Chinese Journal of Reparative and Reconstructive Surgery

摘　　要：目的探讨煅烧异种骨材料的生物相容性,为其应用于临床提供实验依据。方法取健康成年牛松质骨,采用脱蛋白、高温煅烧方法制备煅烧异种骨。将煅烧异种骨材料浸提液与L929细胞体外共同培养,于第2、4、7天评价其细胞毒性。L929细胞接种至煅烧异种骨,培养4 d后扫描电镜观察细胞在材料表面贴附及增殖情况,评价其细胞相容性。于10只新西兰大白兔双侧后肢胫骨钻孔,左侧孔内植入煅烧异种骨(实验组),右侧植入羟基磷灰石生物陶瓷(对照组);术后4、26周取材行大体观察及组织学观察,评价煅烧异种骨生物相容性。结果细胞毒性实验显示,煅烧异种骨浸提液细胞毒性为0~1级。细胞相容性实验显示,L929细胞在煅烧异种骨表面贴附良好,并向孔隙内部生长。体内植入实验显示4周时两组均有轻度或中度炎性反应,可见新骨形成;26周时两组均无炎性反应且有不同程度新骨形成。结论煅烧异种骨具有良好生物相容性,有望用于临床骨缺损修复。Objective To investigate the biocompatibility of true bone ceramic （TBC） and provide experimental basis for clinic application. Methods TBC was prepared from healthy adult bovine cancellous bone by deproteinization and high temperature calcinations. Mouse fibroblast cell line （L929 cells） were cultured with the leaching liquor of TBC in vitro, and the cytotoxicity was evaluated at 2nd, 4th, and 7th days. L929 cells were inoculated into the TBC and cultured for 4 days. The cell adhesion and proliferation on the surface of the TBC were observed by scanning electron microscopy, and evaluated the cell compatibility of TBC. Ten New Zealand white rabbits were divided into 2 groups, and drilled holes at the tibia of both hind limbs. TBC and hydroxyapatite （HA） were implanted into the left side （experimental group） and the right side （control group）, respectively. And the biocompatibility of TBC was evaluated by general observation and histological observation at 4 and 26 weeks after implantation. Results Cytotoxicity test showed that the cytotoxicity level of leaching liquor of TBC was grade 0-1. Cell compatibility experiments showed that the L929 cells adhered well on the surface of TBC and migrated into the pores. The implantation test in vivo showed that experimental group and control group both had mild or moderate inflammatory response at 4 weeks, and new bone formation occurred. At 26 weeks, there was no inflammatory reaction observed in both groups, and new bone formation was observed in varying degrees. Conclusion TBC have good biocompatibility and can be used to repair bone defect in clinic.

关 键 词：煅烧异种骨 细胞毒性 生物相容性 

分 类 号：R318.08[医药卫生—生物医学工程] R68[医药卫生—基础医学]