A Sex-linked Microsatellite Marker Reveals Male Heterogamety in Quasipaa boulengeri(Anura: Dicroglossidae)  

作　　者：Siqi YUAN Yun XIA Xiaomao ZENG 

机构地区：[1]Department of Herpetology,Chengdu Institute of Biology,Chinese Academy of Sciences [2]University of Chinese Academy of Sciences

出　　处：《Asian Herpetological Research》2017年第3期184-189,共6页亚洲两栖爬行动物研究（英文版）

基　　金：supported by the National Science Foundation of China(No.31272282,No.31572241 and No.31401960)

摘　　要：Few amphibians possess morphologically distinguishable heteromorphic sex chromosomes.The classic indirect method is a time-consuming and resource-demanding task to identify the heterogametic sex.Here we have taken advantage of a sex-specific microsatellite marker,by amplifying a large number of samples of known male and female individuals from various populations,to reveal a homogeneous pattern of male heterogamety in Quasipaa boulengeri.The identification of the heterogametic sex will help interpreting the evolution of sex determination.Besides relevance for evolutionary studies of sex determination,the sex-linked markers have potential for addressing practical issues in conservation biology because the sex reversal that caused by anthropogenic endocrine disruptors is considered as a reason for amphibian decline.Few amphibians possess morphologically distinguishable heteromorphic sex chromosomes.The classic indirect method is a time-consuming and resource-demanding task to identify the heterogametic sex.Here we have taken advantage of a sex-specific microsatellite marker,by amplifying a large number of samples of known male and female individuals from various populations,to reveal a homogeneous pattern of male heterogamety in Quasipaa boulengeri.The identification of the heterogametic sex will help interpreting the evolution of sex determination.Besides relevance for evolutionary studies of sex determination,the sex-linked markers have potential for addressing practical issues in conservation biology because the sex reversal that caused by anthropogenic endocrine disruptors is considered as a reason for amphibian decline.

关 键 词：microsatellites sex-specific marker male heterogamety Quasipaa boulengeri 

分 类 号：Q953[生物学—动物学]