硅包银纳米颗粒的制备及其光散射法检测腺苷  

Preparation of Ag@SiO_2 Nanoparticles and Detection of Adenosine by Light Scattering

作　　者：李靖云[1] 韩国斌 刘建萍 李原芳[1] LI Jing-yun HAN Guo-bin LIU Jian-ping LI Yuan-fang(Education Ministry Key Laboratory on Luminescence and Real-Time Analysis/School of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, China Chongqing Forty-Eighth Middle School, Chongqing 400700, China)

机构地区：[1]发光与实时分析教育部重点实验室/西南大学化学化工学院,重庆400715 [2]重庆第48中学,重庆400700

出　　处：《西南大学学报（自然科学版）》2017年第9期113-117,共5页Journal of Southwest University(Natural Science Edition)

基　　金：国家自然科学基金项目(21175109)

摘　　要：将腺苷的核酸适配体设计成两段DNA链,一段修饰在硅包银纳米颗粒上,另一段修饰在磁性颗粒上.利用腺苷与其核酸适配体的特异性结合,通过检测磁性分离后上清液中硅包银纳米颗粒的光散射信号变化,实现了腺苷检测.方法的线性范围为8.0×10^(-6)~5.0×10^(-4)mol/L,线性相关系数(r)为0.981 8,其他的核苷不干扰测定.Silver nanoparticles（AgNPs）have the property of strong localized surface plasmon resonance scattering,but their stability and functionality are inferior.To solve these problems,silica-coated silver（Ag@SiO_2）nanoparticles were prepared by packing a SiO_2 layer on AgNPs.Then a new method was designed for adenosine detection by using Ag@SiO_2NPs with separation of magnetic particles（MPs）.It was found that a linear relationship（ΔILS=666.50＋505.18logc）was obtained between the value ofΔILSand the concentration of adenosine in the range of 5.0×10^（-4）-8.0×10^（-6） mol/L with a correlation coefficient（r） of 0.981 8.Moreover,no obvious effects were detected of other nucleosides on the analysis of adenosine.

关 键 词：硅包银纳米颗粒 磁性颗粒 光散射 腺苷 核酸适配体 

分 类 号：O657[理学—分析化学]