miR-132在神经病理性疼痛传导通路中的表达  

The expression of miR-132 in Neuropathic pain-related signaling pathway

作　　者：施燕渲 朱涛 

机构地区：[1]南京医科大学附属上海松江中心医院,201600

出　　处：《实用医学杂志》2017年第19期3189-3192,共4页The Journal of Practical Medicine

基　　金：上海市松江区科委科技攻关项目(编号:14SJG-Gyy10)

摘　　要：目的通过机械损伤致神经病理痛大鼠模型,检测神经系统不同节段mi R-132定量变化。方法 45只SD大鼠随机平均分为对照组(Native)、假手术组(Shame)和坐骨神经结扎组(BCCI),每组15只。于术前1 d,术后1、7、14 d测定机械痛阈(MWT)。取海马,L4~6脊髓背角、背根神经节并采用RT-PCR定量检测mi R-132的表达。结果 BCCI组MWT在术后7和14 d明显低于Native组及Shame组(P<0.01)。Mi R-132表达量:(1)背根神经节:术后第7天BCCI组和Shame组相比Native组均明显降低(P<0.05);第14天Shame组恢复至术前水平,而BCCI组表达量仍降低(P<0.05)。(2)脊髓背角:术后第7天BCCI组表达量明显增高(P<0.05),第14天BCCI组与Shame组均明显降低(P<0.05)。(3)海马:术后第14天BCCI组表达量明显降低(P<0.01)。结论 mi RNA-132参与了损伤性神经病理痛的发生发展。mi RNA-132在BCCI大鼠模型的疼痛传导通路不同节段不同时间节点表达水平发生显著的变化,不同部位的变化存在明显的差异性。Objective To investigate the miR-132 expression in different segments of the nervous system through mechanical damage induced neuropathic pain rat model. Methods A total of 45 male SD rats （250 - 300 g） were randomly divided into Native, Shame and BCCI groups, with 15 rats in each group. The mechanical with- drawal threshold（MWT）was measured 1 day before BCCI, and 1 d, 7 d, 14 d after BCCI. The expression levels of miR-132 in Hippocampus （HP）, L4-6 spinal dorsal horn （SDH） and dorsal root ganglia （DRG） were measured indi- vidually by reverse transcription-polymerase chain reaction （RT-PCR）. Results Compared with Shame group and Native group, MWT decreased significantly in BCCI group 7 d and 14 d after operation （P 〈 0.01 ）. Compared with the Native group, the expression of miR-132 in DRG decreased in BCCI group and Shame group 7 d after operation （P 〈 0.05）. Rats in shame group recovered by 14 d while BCCI was still decreased significantly （P 〈 0.05）. The miR-132 expression of SDH was upregulated significantly in BCCI group at day 7 （P 〈 0.05）. On the 14 day after operation, the expression of miR-132 was downregulated significantly in BCCI group and Shame group （P 〈 0.05）. BCCI group decreased significantly at 14 d （P 〈 0.01 ）. Conclusions MiR-132 was involved in the regulation of neuropathic pain. The expression of miR-132 has its own characteristics in different segments and time points.

关 键 词：神经病理性疼痛 坐骨神经结扎 miR-132 

分 类 号：R402[医药卫生—临床医学]