基于超高效液相色谱-四极杆飞行时间质谱的缺血性脑卒中的代谢组学研究  

UPLC-QTOF-MS-based Metabolomics Study on Ischemic Stroke Patients

作　　者：黄玉 谷彩云[2] 吴翰钟[3] 夏晓爽[1] 李新[1] 

机构地区：[1]天津医科大学第二医院神经内科,天津300211 [2]天津中医药大学中药学院,天津300193 [3]天津大学精密测试技术及仪器国家重点实验室,天津300072

出　　处：《高等学校化学学报》2017年第10期1742-1750,共9页Chemical Journal of Chinese Universities

基　　金：国家自然科学基金(批准号:51105274);天津市自然科学基金(批准号:16JCYBJC25500);天津市科学技术委员会支撑项目(批准号:12ZCZDSY03100)资助~~

摘　　要：采用基于超高效液相色谱-四极杆飞行时间质谱的代谢组学方法,研究缺血性脑卒中患者和健康人群的血浆,分析了缺血性脑卒中的生物标志物.实验收集30例缺血性脑卒中患者和17例健康志愿者的血浆样品,采用主成分分析和正交偏最小二乘判别分析,研究了缺血性脑卒中患者组和健康对照组血浆中的代谢物差异,并进行了代谢通路分析.实验结果表明,患者组血浆中的二氢神经鞘氨醇、植物鞘氨醇等物质含量升高,谷氨酰胺、焦谷氨酸和2-酮丁酸等物质含量降低.结果表明,脑卒中不仅影响了鞘脂类和氨基酸的代谢,还对能量代谢产生了显著的影响.Ultra-performance liquid chromatography with quadrupole-time-of-flight mass spectrometry（ UPLCQTOF-MS） based metabolomics was used to study the metabolism profiling of the plasma from the patients with ischemic stroke,aiming to seek the potential biomarkers of ischemic stroke. 30 patients with ischemic stroke and 17 healthy controls were enrolled. Principal component analysis and orthogonal partial least squares discriminate analysis were used to investigate the metabolic variations between the patients with ischemic stroke and healthy controls. Metabolic pathways were analyzed. Seven biomarkers were obtained. The levels of sphinganine and phytosphingosine were increased,and the levels of glutamine,pyroglutamic acid and 2-ketobutyric acid were decreased. The results of the pathway analysis showed that sphingolipid metabolism and amino acid metabolism were perturbed,and the energy metabolism was also disturbed.

关 键 词：代谢组学 超高效液相色谱-四极杆飞行时间质谱 脑卒中 血浆 

分 类 号：O657[理学—分析化学]