双氰基二苯代乙烯型双光子荧光汞离子探针  

Dicyanostilbene-derived Two-photon Fluorescence Probe for Mercury Ions

作　　者：黄池宝 潘淇[1] 陈晓远 赵光练[1] 陈华仕 梁兴 吕国岭 

机构地区：[1]遵义师范学院化学化工学院,遵义563002 [2]韶关大学农业科学与工程学院,韶关512005

出　　处：《高等学校化学学报》2017年第10期1751-1756,共6页Chemical Journal of Chinese Universities

基　　金：贵州省高层次创新型人才培养计划项目--"百"层次人才(批准号:黔科合平台人才[2016]5683);国家自然科学基金(批准号:21562050);贵州省第八批科技创新人才团队建设专项基金[批准号:黔科合人才团队(2015)4007];贵州省科学技术基金项目(批准号:黔科合J字[2015]2146);贵州省教育厅自然科学重点研究项目(批准号:黔教合KY字[2014]296);贵州省教育厅省级本科教学工程建设项目(批准号:黔教[2014]JXGChcb);遵义市"15851人才精英工程"项目[批准号:(2015)4007];遵义市红花岗区科学技术项目(批准号:遵红科合社字[2015]18)资助~~

摘　　要：分别以双氰基二苯代乙烯(DCS)和双[2-(2-羟乙基硫基)乙基]氨(HSA)为双光子荧光团和汞离子受体,合成了双光子荧光汞离子探针(DHg),并对其结构进行了分析.实验结果表明,DHg在甲苯、乙腈和水中的荧光量子产率(Φ)分别为0.78,0.42和0.20,对汞离子的络合常数通过单、双光子荧光滴定分别拟合为lg K=5.47±0.02和lg K=5.34±0.02.DHg在水溶液中对汞离子具有优良的选择性和高的灵敏性,可用于中性环境中汞离子的检测.DHg的双光子吸收截面(δTPA)在水溶液中高达840 GM,可用于细胞中汞离子的检测与成像.A novel water-soluble two-photon fluorescent probe for Hg2＋（ DHg） derived from dicyanostilbene（ DCS） as a two-photon fluorophore and bis[2-（ 2-hydroxyethyl sulfanyl） ethyl]amino group（ HAS） as a novel Hg2＋ligand was developed,and its structure was analyzed and identified. The results of UV-Vis absorption and absorption-titration experiments,one-and two-photon excited fluorescence experiments,as well as cellimaging and tissue-imaging experiments showed that the probe has a very high sensitivity and selectivity for the detection of Hg2＋in water. It can be used to detect trace Hg2＋in neutral or basic aqueous solution（ p H = 7—12）,and its binding constant（ lg K） for Hg2＋was determined from the one-and two-photon fluorescence-titration curves to be lg K = 5. 47±0. 02 and lg K = 5. 34±0. 02,respectively. Nitrogen atom property and the sulfur atom number on the ionophore are believed to be responsible of the unique selectivity of probe DHg for Hg2＋. The fluorescent quantum yields of DHg in toluene,acetonitrile and water are 0. 78,0. 42 and 0. 20,respectively,and its two-photon absorption cross-section（ δTPA） is as high as 840 GM in aqueous solution. The probe could selectively detect free Hg2＋ions in live cells without interference from other metal ions and the membranebound probes. Undoubtedly,the probe is an ideal two-photon excited fluorescence bioimaging candidate.

关 键 词：双光子荧光探针 汞离子 活体成像 双光子吸收截面 

分 类 号：O657.3[理学—分析化学]