基于脑区特异性不同剂量冰片与川芎配伍抗脑缺血的研究  

Research on the synergy of different doses of borneol and Chuanxiong Rhizoma on the anti-ischemnia effect in different brain regions

作　　者：喻斌[1,2] 沈祥春 梁涛 黄仕文[2] 杨丽 程海波[2] 阮鸣 

机构地区：[1]南京中医药大学江苏省中药药效与安全性评价重点实验室/部省共建中药药理实验室,江苏南京210023 [2]南京中医药大学转化医学研究中心/国家中医药管理局名医验方评价与转化重点研究室,江苏南京210023 [3]贵州医科大学药学院,贵州贵阳550025 [4]南京晓庄学院江苏省高校特殊生物质废弃物资源化利用重点建设实验室,江苏南京211117

出　　处：《中国医院药学杂志》2017年第18期1792-1796,共5页Chinese Journal of Hospital Pharmacy

基　　金：国家自然科学基金项目(编号:81573713;81001651);江苏省自然科学基金(编号:BK20151564;BK20150088);江苏省大学生创新性实训项目;江苏省高校重点建设实验室项目(编号:苏教科[2016]8);贵州省科技支撑计划(编号:黔科合[2016]支撑2826)

摘　　要：目的:观察不同剂量冰片配伍川芎对不同脑区缺血的保护作用。方法:复制大鼠全脑缺血-再灌注模型,观察不同剂量冰片(0.01,0.02,0.04,0.08,0.16,0.32,0.64,1.28 g·kg-1)联合川芎对大鼠皮层、海马、下丘脑和纹状体四个脑区梗死率,脑组织IL-1β、IL-6、TNF-α含量和组织学评分的影响。结果:川芎单用能显著改善皮层区TNF-α含量。其配伍冰片后对四个脑区的梗死率,IL-1β、IL-6、TNF-α含量和组织学评分均表现出更好的疗效。另外针对不同脑区,两药配伍疗效比较为皮层>海马、纹状体>下丘脑。针对冰片剂量,两药配伍疗效比较为0.08 g·kg^(-1)>0.16 g·kg^(-1)>0.32 g·kg^(-1)。结论:川芎和冰片配伍可以对缺血脑组织产生脑区特异性改善,而且这一改善作用与冰片剂量密切相关。OBJECTIVE To investigate the enhancement effects of borneol at different doses on the anti-ischemnia of Chuanxiong Rhizoma（CR）in different brain regions.METHODS After the model of cerebral ischemia-reperfusion was established,the effect of CR combined with different doses of borneol was evaluated based on brain infarct,levels of IL-1β,IL-6,TNF-αand nissl staining.RESULTS Monotherapy of CR only improved TNF-α content in cortex,but the combined therapy of CR and borneol produced better improvements in infarct area,levels of IL-1β,IL-6,TNF-α and nissl staining in each brain region.Moreover,the therapeutic effects according to the brain regions were cortex〉hippocampus,striatum〉hypothalamus,the therapeutic effects according to the borneol dose were 0.08 g·kg^-1〉0.16 g·kg^-1〉0.32 g·kg^-1.CONCLUSION The combining therapy of CR and borneol induces a better improvement than monotherapy of CR in a region-specific manner,which is closely related to the dose of borneol.

关 键 词：川芎 冰片 脑区特异性 脑缺血 剂量 

分 类 号：R285.5[医药卫生—中药学]