UPLC测定豌豆尖中槲皮素和山奈酚的含量  

Determination of quercetin and kaempferol in pea shoots by UPLC

作　　者：曹晓华[1] 程祖锌[1] 林兆娜 程先骄 郑金贵 CAO Xiao-hua CHENG Zu-xin LIN Zhao-na CHENG Xian-jiao ZHENG Jin-gui(Fujian Engineering Technology Research Center for Breeding and Utilization of Special Crops, Key Laboratory of Ministry of Education for Genetics, Breeding and Multiple Utilization of Crops, College of Crop Science, Fujian Agriculture and Forestry University, Fuzhou 35000)

机构地区：[1]福建农林大学作物遗传育种与综合利用教育部重点实验室福建省特种作物育种与利用工程技术研究中心,福州350002

出　　处：《食品科技》2017年第9期284-287,共4页Food Science and Technology

基　　金：国家科技支撑计划项目(2013BAD01B05);福建农林大学科技创新平台建设项目(PTJH13001;PTJH12015)

摘　　要：目的:采用超高效液相色谱法(UPLC)测定豌豆尖中类黄酮水解产物槲皮素和山奈酚的含量。方法:采用Waters ACQUITY UPLC BEH Shield RP18色谱柱(100 mm×2.1 mm,1.7μm),以乙腈-0.1%冰醋酸水溶液(60:40)为流动相,流速为0.4 m L/min,槲皮素检测波长为371 nm,山奈酚检测波长为366 nm,柱温为30℃,进样量2.0μL。结果:槲皮素和山奈酚在1.5 min时间内达到基线分离,分别在(0.03~0.18)mg/m L、(0.015~0.15)mg/m L范围内与峰面积线性关系良好,平均回收率分别为101.37%、100.64%,RSD分别为0.84%、0.37%。结论:该方法快速、准确、稳定,可用于豌豆尖中槲皮素和山奈酚的含量测定。Objective： To establish an UPLC method for simultaneous determination of quercetin and kaempferol in flavonoids hydrolysate from pea shoots. Methods： The sample solution was separated on a Waters ACQUITY UPLC BEH Shield RP18（100 mm×2.1 mm, 1.7 μm） column with acetonitrile-0.1% glacial acid solution（60：40） as mobile phase at a flow rate of 0.4 m L/min. The detection wavelength of quercetin was set at 371 nm and kaempferol＇s detection wavelength was set at 366 nm, the column temperature was set at 30 ℃ and the injection volume was 2 μL. Results： Quercetin and kaempferol were well separated in 1.5 minutes and had good linear relationship within the range of（0.03~0.18）mg/m L and（0.015~0.15）mg/m L respectively. The average recovery rate of quercetin and kaempferol were 101.37% and 100.64% respectively, the RSD were 0.84% and 0.37% respectively. Conclusion： The method is fast, accurate and stable for the determination of quercetin and kaempferol in pea shoots.

关 键 词：超高效液相色谱 豌豆尖 槲皮素 山奈酚 含量测定 

分 类 号：O657.72[理学—分析化学]