IL-32及IL-5对哮喘小鼠气道黏液高分泌的影响及地塞米松的干预作用  

Influence of IL-32 and IL-5 on airway mucous hypersecretion and dexamethasone intervention effect in asthmatic mice

作　　者：郑飞彦 代大华 王莉[1] 杨红霞[1] 张建勇[1] 

机构地区：[1]遵义医学院附属医院呼吸二科,贵州遵义563003 [2]遵义医学院附属医院重症医学科,贵州遵义563003

出　　处：《现代医药卫生》2017年第19期2912-2914,共3页Journal of Modern Medicine & Health

基　　金：贵州省科技计划基金资助项目(黔科合SY字[2008]3056)

摘　　要：目的探讨白介素32(IL-32)及IL-5对哮喘小鼠气道黏液高分泌的影响及地塞米松的干预作用。方法将清洁级雄性BALB/c小鼠30只随机分成生理盐水(NS)对照组(NS组)、哮喘模型(AS)组(AS组)、AS+地塞米松(DEX)干预组(AS+DEX组),各10只。AS组第1、13天予0.2 mL卵清蛋白(OVA)混悬液腹腔注射致敏,第19天予5%OVA溶液雾化激发,每次30 min,连续5 d。AS+DEX组在雾化激发前30 min予地塞米松(2 mg/kg)腹腔注射干预,连续5 d,余同AS组。NS组用生理盐水进行致敏和激发。用酶联免疫吸附试验检测小鼠肺泡灌洗液(BALF)中细胞因子IL-32吸光度(OD)和IL-5含量,苏木精-伊红染色及阿尔辛蓝-过碘酸雪夫(AB-PAS)染色肺组织,逆转录-聚合酶链反应检测Muc5ac mRNA表达,免疫组织化学法检测Muc5ac及IL-32蛋白表达。结果 AS组小鼠BALF中IL-5含量、IL-32 OD、AB-PAS阳性表达面积、Muc5ac mRNA,Muc5ac及IL-32蛋白表达均较NS组明显增高,AS+DEX组上述指标均较AS组明显降低,但仍高于NS组,差异均有统计学意义(P<0.05)。结论 IL-32和IL-5可促进哮喘小鼠气道黏蛋白Muc5ac高表达,DEX可能通过下调二者的表达抑制黏液高分泌。Objective To investigate the influence of IL-32 and IL-5 on airway mucus hypersecretion in asthmatic mice and dexamethasone（DEX） interventional effect. Methods Thirty clean class male BALB/c mice were randomly divided into the normal saline（NS） control group（NS group）,asthmatic model（AS） group（AS group） and AS＋DEX intervention group（AS＋DEX group）,10 cases in each group. The AS group was given ovalbumin（OVA） suspension fluid 0.2 mL by intraperitoneal injection on1,13 d for causing sensitization and 5% OVA solution on 19 d for conducting atomization stimulation, 30 min per time for successive 5 d. The AS＋DEX group was interfered by intraperitoneal injection of DEX 2 mg/kg at 30 min before atomization stimulation for successive 5 d. The remaining was same to that in the AS group. The NS group was given normal saline for causing sensitization and stimulation. The levels of cytokine IL-32 absor bance（OD） and IL-5 content in bronchoalveolar lavage fluid（BALF） were determined by ELISA,the lung tissues were observed by hematoxylin-eosin（HE） staining and alcian blue/periodic acid Schiff（ABPAS） staining,the expression of Muc5ac mRNA in lung tissue was detected by RT-PCR,the expressions of mucin 5ac（Muc5ac）and IL-32 protein were measured by using the immunohistochemical method. Results The IL-5 content and IL-32 OD,AB-PAS positive expression area, and expressions of Muc5ac mRNA,Muc5ac and IL-32 protein in BALF of the AS group were all significantly increased compared with those in the NS group,while the above indicators in the AS ＋DEX group were significantly decreased compared with the AS group,but which were still higher than those in the NS group,the differences were statistically significant（P〈0.05）. Conclusion IL-32 and IL-5 can promote airway Muc5ac protein high expression in mice with asthma,and dexamethasone may inhibit mucous hypersecretion by down-regulating their expressions.

关 键 词：哮喘 气管 白细胞介素5 白细胞介素32 地塞米松 黏蛋白5AC 小鼠 

分 类 号：R978.15[医药卫生—药品]