高效分离提取蒜氨酸方法研究  

Study on efficient separation and extraction method of alliin

作　　者：李明强[1] 胡铭 宋删删 罗娅[3] 韩志俊[1] 李恒梅 

机构地区：[1]遵义医学院微生物学与免疫学实验室,贵州遵义563000 [2]遵义医学院临床医学系,贵州遵义563000 [3]遵义医学院公共卫生管理学院,贵州遵义563000

出　　处：《现代医药卫生》2017年第19期2937-2940,共4页Journal of Modern Medicine & Health

基　　金：遵义医学院大学生创新课题(遵医[2015]5035)

摘　　要：目的建立一种方法简单、成本低廉、高效、高纯度、安全、无毒、易于推广的蒜氨酸分离提取方法。方法在保持蒜酶活性条件下匀浆大蒜,4℃冰箱透析,蒜氨酸沉淀。采用化学方法(磺基水杨酸试验、双缩脲试验)、物理方法(紫外光谱吸收实验、液相色谱分析)、相对分子质量(液相色谱-质谱联合技术分析)、分子结构(红外光谱分析、核磁共振图谱分析)等对大蒜中的蒜氨酸进行鉴定。结果样品经化学方法、物理方法、相对分子质量、分子结构等鉴定均显示为蒜氨酸成分。结论该研究为蒜氨酸的开发、利用提供了最为简便的提取方法,具有稳定、可靠、高效、高纯度、成本低廉等优点。Objective To establish a method for separating and extracting alliin,which has low cost,high efficiency,high purity,safety and non-toxicity,and is simple and easy to promote. Methods Under the condition for keeping the allinase activity,the garlic homogenate was dialyzed at 4 ℃ refrigerator. Alliin was identified by adopting the chemical methods（sulfosalicylic acid test,biuret test）, physical methods（ultraviolet spectrum adsorption test,liquid chromatography）,relative molecular mass methods（liquid chromatography-mass spectrum combined analysis technique） and molecular structure methods（ultraviolet spectrum analysis,magnetic resonance imaging spectrum analysis）. Results The sample was identified as alliin composition by chemical,physical,relative molecular weight,molecular structure methods. Conclusion The study provides the simplest alliin extraction method for its development and utilization,which has the advantages of reliability,stability,high efficiency,high purity and low cost.

关 键 词：大蒜氨酸/分离和提纯 氨基酸类 透析 方法 

分 类 号：R282.71[医药卫生—中药学]