德国镜鲤抗病选育群体的亲子鉴定及遗传结构分析  

Paternity Identification and Genetic Structure Analysis of Disease-Resistant Breeding Population of German Mirror Carp(Cyprinus carpio)

作　　者：贾智英[1] 白姗姗[1,2] 李池陶 李保[3] 石连玉 

机构地区：[1]中国水产科学研究院黑龙江水产研究所,哈尔滨150070 [2]上海海洋大学水产与生命技术学院,上海201306 [3]宽甸满族自治县水产苗种管理站,宽甸118299

出　　处：《基因组学与应用生物学》2017年第9期3735-3741,共7页Genomics and Applied Biology

基　　金：黑龙江所基本业务费"鲤抗疱疹病毒F3选育研究"(HSY201401);国家"十二五"科技支撑计划项目"重要水产种质资源群体构建及遗传评价方法研究(2012BAD26B01);国家大宗淡水鱼类产业技术体系北方鲤鱼种质资源与育种项目(CARS-46-02);2015年度黑龙江省领军人才梯度后备带头人资助项目共同资助

摘　　要：为选育抗疱疹病毒(Cy HV-3)的新品种德国镜鲤,本研究在F_1抗病家系基础上构建10个家系分A、B两组混养。从193对引物中分别筛选3对高多态性微卫星引物进行亲子鉴定及遗传结构分析。结果显示:(1)两组中3个位点的累积排除率为99.99%、99.98%,亲子代配对率为100%(p=0.05),说明利用3个高多态性位点就可达到亲子鉴定的目的。(2)家系鉴定成活率为0%~60.21%。其中1#、8#、9#、10#家系比对照组提高43.51%、22.00%、11.64%、8.45%,可用于F_3选育。(3)1#、8#、9#、10#4个家系的遗传多样性指标为:Ae为3.507 7~4.033 2、Ho为0.818 1~0.965 8、He为0.7062~0.7386,遗传多样性水平较高。(4)除9#家系的HLJ3939位点显著偏离Hardy-Weinberg平衡(p<0.05)外,其他位点均极显著偏离Hardy-Weinberg平衡(p<0.01),说明所选位点均经过了选择作用。(5)1#、8#、9#、10#4个家系FST为0.057 3~0.115 6,8#、9#、10#3个家系间的Nei氏遗传距离均值为0.515 0,说明4个家系具有较高遗传潜力。因此,本研究成功筛选出4个成活率较高且具有较高遗传潜力的家系,以用于下一代选育。In order to breed new species of German mirror carp which was resistant to Cy HV-3,we constructed ten families and cultured them mixedly into two groups（A and B） based on the F_1 disease-resistant family.We screened out three pairs of high polymorphic microsatellite primers from 193 pairs of primers for paternity identification and genetic structure analysis.The results show that：（1） Cumulative elimination rate of 3 loci in the two groups were 99.99% and 99.98%,respectively.Parents-offspring pair rate was 100%（p=0.05）,which indicated that three high polymorphisms loci could reach the requirements of paternity identification.（2） The survival rates of each family were 0% ~60.21%.The survival rate of family 1#,8#,9# and 10# increased by43.51%,22%,11.64% and 8.45% compared to the control group,and they could be used for F_3 breeding.（3）The genetic diversity indexes of 1#,8#,9# and 10# these four families：Ae ranged from 3.507 7 to 4.033 2,Ho from0.818 1 to 0.965 8,and He from 0.706 2 to 0.738 6.The genetic diversity levels of those families were relatively high.（4） Only the HLJ3939 locus of 9# family was significantly deviated from Hardy-Weinberg equilibrium（p〈0.05）.Other loci were extremely significantly deviated from Hardy-Weinberg equilibrium（p〈0.01）.This meant that all the loci had experienced selection.（5） The FST of 1#,8#,9# and 10# family ranged from 0.057 3 to 0.115 6,and the mean value of Nei＇s genetic distance among family 8#,9# and 10# was 0.515 0.It showed that those four families had relatively high genetic breeding potential.Therefore,we successfully screened out 4 families with relatively high survival rate and genetic breeding potential for the next generation of breeding.

关 键 词：德国镜鲤 遗传结构 锦鲤疱疹病毒3型 

分 类 号：S917.4[农业科学—水产科学]