刺五加鲨烯环氧酶基因DNA和启动子的克隆与分析  

Cloning and Analysis of DNA and Promoter of SE Gene in Eleutherococcus senticosus

作　　者：尤鹏升 国红玉 李志栋 龙月红 邢朝斌 

机构地区：[1]华北理工大学生命科学学院,唐山063000

出　　处：《基因组学与应用生物学》2017年第9期3842-3847,共6页Genomics and Applied Biology

基　　金：国家自然科学基金项目(31570683);河北省教育厅资助科研项目(QN2014102);华北理工大学培育基金(SP201508);华北理工大学大学生创新创业训练计划(X2016045)共同资助;河北省大学生创新创业训练计划(201610081045)

摘　　要：本研究以刺五加鲨烯环氧酶(squalene epoxidase,SE)基因的c DNA序列为基础设计特异性引物,利用PCR和TAIL-PCR扩增SE DNA的全长序列,结合Plant CARE等软件,对启动子中的作用元件进行生物信息学分析。克隆得到长6 307 bp的刺五加SE DNA和启动子序列,包含8段外显子、7段内含子及两端的非翻译区,编码554个氨基酸;上游启动子序列长1 907 bp,含36个TATA-box,27个CAAT-box,此外还有茉莉酸甲酯调控元件、脱落酸调控元件以及光调控元件等多种顺势作用元件,表明SE基因表达受植物激素、光照以及温度等多种因素的调控。In this paper,based on the c DNA sequence of squalene epoxidase（SE） gene in E.senticosus,we designed specific primers.PCR and TAIL-PCR were used to amplify DNA full-length sequence of SE.Then combined with Plant CARE and other softwares,bioinformatics analysis was performed on the functional elements in the promoter.DNA and promoter sequence of E.senticosus SE gene with 6 307 bp was cloned,containing 8exons,7 segment introns and untranslated regions at both ends and encoding 554 amino acids.The upstream promoter sequence was 1 907 bp long,containing 36 TATA-box and 27 CAAT-box.Besides,it also contained a variety of cis-acting elements,such as jasmonic acid regulatory element,abscisic acid regulatory element,and light regulatory elements,which indicated that the expression of SE gene was regulated by many factors,such as plant hormone,light and temperature.

关 键 词：刺五加 SE基因 启动子 克隆 调控元件 

分 类 号：Q943.2[生物学—植物学] S567.19[农业科学—中草药栽培]