蝙蝠葛碱对阿尔茨海默病小鼠海马晚期糖基化终产物受体和核转录因子-κBp65的影响  

The effects of dauricine on receptor for advanced glycation end products and nuclear transcription factor-κBp65 of the hippocampus in Alzheimer's disease mice

作　　者：张英博[1] 费洪新[1] 郭家[1] 兴桂华[1] 潘思文[1] 张晓杰[1] 周忠光[2] ZHANG Ying-Bo FEI Hong-Xin GUO Jia et al(Qiqihar Medical University, Qiqihaer 161006, Heilongjiang, China)

机构地区：[1]齐齐哈尔医学院,黑龙江齐齐哈尔161006 [2]黑龙江中医药大学

出　　处：《中国老年学杂志》2017年第19期4697-4700,共4页Chinese Journal of Gerontology

基　　金：国家自然科学基金(81373777;81173599);齐齐哈尔医学院院内科研博士专项基金(QY2016B-21;QY2016B-26);黑龙江省教育厅科学研究项目(12531788)

摘　　要：目的探讨蝙蝠葛碱(DAU)对阿尔茨海默病(AD)小鼠海马晚期糖基化终产物受体(RAGE)和核转录因子(NF)-κBp65的影响。方法雄性小鼠随机分成对照组、模型组、多奈哌齐组(0.001 g/kg)及DAU高、中、低剂量组(2.4、1.2、0.6 g/kg)。小鼠双侧海马微量注射β-淀粉样蛋白(Aβ)_(1~42)(2 g/L)和腹腔注射D-半乳糖(180 mg/kg)诱导AD小鼠,连续治疗35 d后取材。采用Morris水迷宫(MWM)检测AD小鼠学习记忆能力,酶联免疫吸附(ELISA)检测海马白细胞介素(IL)-1β、IL-6含量,免疫组化(IHC)、实时定量(RT)-PCR和Western印迹检测海马RAGE和NF-κBp65表达。结果与模型组比较,DAU高、中剂量明显改善AD小鼠学习记忆能力,明显降低海马IL-1β、IL-6、RAGE和NF-κBp65水平(均P<0.05)。结论 DAU通过抑制海马RAGE和NF-κBp65表达来改善AD小鼠学习记忆能力,以此在AD治疗中发挥重要作用。Objective To explore the effects of dauricine（ DAU） on receptor for advanced glycation end products（ RAGE） and nuclear transcription factor（ NF）-κBp65 of the hippocampus in Alzheimer＇s disease（ AD） mice. Methods Male mice were randomly divided into control,model,donepezil（ 0.001 g/kg）,DAU high-dose（ 2.4 g/kg）,DAU medial-dose（ 1.2 g/kg）,DAU low-dose（ 0.6 g/kg） groups.The AD model mice were induced by microinjection of incubated amyloid beda91 ~ 42）（ 2 g/L） into the dorsal blade of and intraperitoneal injection of D-galactose（ 180 mg/kg） in the dentale gyrus in the bilateral hippocampus. Morris water maze was used to observe the learning and memory ability of AD mice. Enzyme-linked immunosorbent assay（ ELISA） was used to determine the contents of IL-1β and IL-6. By immunohistochemistry（ IHC）,real time（ RT）-PCR and Western bolt were used to test the contents of RAGE and NF-κBp65 of the hippocampus in AD mice.Results Compared with those of model group,DAU high-dose and medial-dose significantly improved the learning and memory ability of AD mice（ P〈0.05）. DAU high-dose and medial-dose decreased the levels of IL-1β,IL-6,RAGE and NF-κBp65 in the hippocampus（ P〈0.05）.Conclusions DAU could improve the ability of learning and memory of AD mice. DAU plays a certain role in the treatment of AD through inhibiting RAGE and NF-κBp65.

关 键 词：蝙蝠葛碱 阿尔茨海默病 晚期糖基化终产物受体 

分 类 号：R285[医药卫生—中药学]