应用微孔板芯片分析机采血小板常温保存过程中凋亡相关miRNA的表达改变  

Identification of differentially expressed apoptosis-associated miRNA in room temperature-stored apheresis platelets by miRNA plate array

作　　者：陈倩[1] 李朝红[2] 谭琳[3] 张志亮[1] 唐秋萍[1] 许开宁 符策瑛 

机构地区：[1]海南省血液中心,海南海口570311 [2]中山大学中山医学院 [3]中国热带农业科学院海口实验站

出　　处：《中国输血杂志》2017年第8期893-896,共4页Chinese Journal of Blood Transfusion

基　　金：海南省自然科学基金(814366);海南省卫计委医学科研课题资助项目(2013052)

摘　　要：目的分析机采血小板在常温保存过程中凋亡相关miRNA的表达改变。方法应用miRNA微孔板芯片技术研究常温保存机采血小板在不同保存时间凋亡相关miRNA的差异表达谱,采用生物信息学软件预测发生差异表达的miRNA可能的靶基因及相关生物学功能。结果与新鲜血小板相比,常温保存7 d的血小板,有7种miRNA发生了显著变化。其中miR-16、Let-7b、miR-26和Let-7c的表达上调,miR-7、miR-145和miR-197的表达下调。结论筛选出的差异表达miRNA可能参与了血小板的保存损伤过程。Objective To explore the variant expression profile of apoptosis-related microRNA （miRNA） in apheresis platelets during room temperature storage. Methods miRNA plate array was used to investigate the apoptosis-associated miRNA profile of apheresis platelets after different storage periods at room temperature. Various biological information soft- ware and databases were utilized to predict target genes and molecular functions of the selected miRNA. Results After room temperature storage for 7 days, as compared with fresh platelets, seven miRNA demonstrated high expression such as miR- 16, Let-7b, miR-26, and Let-7c, which were significantly upregulated, and miR-7, miR-145 and miR-197, which were downregulated. Conclusion The differentially expressed miRNA in this study may play an important role in the development of platelet storage lesion.

关 键 词：miRNA微孔板芯片 机采血小板 凋亡 MIRNA 

分 类 号：R457.1[医药卫生—治疗学] R331.143[医药卫生—临床医学]