无偿献血者HIV检测和归队情况分析  

Voluntary blood donors HIV Testing and regression analysis

作　　者：王丽梅[1] 池泉[1] 林授[1] 何小兰 

机构地区：[1]福建省血液中心,福建福州350004

出　　处：《中国输血杂志》2017年第8期901-903,共3页Chinese Journal of Blood Transfusion

摘　　要：目的分析无偿献血者血液HIV病原学筛查及归队检测结果,为献血者HIV病原学筛查、确证和归队策略提供数据支持。方法对2012年4月16日-2016年6月30日,福建省血液中心无偿献血者344 515名进行抗-HIV ELISA和NAT检测。抗-HIV ELISA反应性和(或)HIV RNA反应性送HIV确证实验室确证。WB确证为抗-HIV阴性且HIV RNA无反应性献血者,在献血半年后,可归队检测。结果血液筛查共检出献血者HIV项目反应性361例。其中抗-HIV ELISA反应性合并HIV RNA反应性98例,WB抗-HIV确证结果为阳性90例,抗-HIV不确定后失访8例;抗-HIV ELISA无反应性但HIV RNA反应性1例,WB抗-HIV确证阳性;抗-HIV ELISA反应性但NAT无反应性262例,WB抗-HIV确证结果阴性259例,抗-HIV不确定后失访3例。抗-HIV确证阳性的91例献血者,WB反应带型均为HIV-1型。29名归队体检献血者,10名允许归队,其中7名再次献血合格。结论福州地区献血者人群中HIV感染以1型为主;HIV RNA反应性对判定献血者HIV感染状态具有较高的特异性;采用NAT和ELISA联合筛查检测,以及归队策略能够保障献血者的献血权利和血液安全。Objective This study aims to analyze the results of the screening and regression tests on voluntary blood do- nors for HIV detection and to provide data support for screening, verification and reentry strategy for blood donors. Methods The donated blood samples were acquired from Fujian blood center, dating from April 16th, 2012 to June 30th, 2016. Anti-HIV ELISA and NAT tests were performed on these samples in 344 515 cases. Anti-HIV ELISA reactive and （or） HIV RNA reactive donor samples were then confirmed by HIV confirmatory laboratory. Donors confirmed by western blot （WB） as anti-HIV negative and HIV RNA non-reactive will be considered valid to rejoin after six months.Results 361 donors were HIV reactive. 98 out of the 361 were confirmed anti-HIV ELISA reactive and 90 of which are determined positive in the WB test while 8 cases lost contact with the donors; 1 sample was tested to be anti-HIV ELISA non-reactive but HIV RNA reac- tive; 1 case was confirmed positive by WB; There were 262 anti-HIV ELISA reactive but HIV RNA non reactive cases, and 259 cases were confirmed negative by WB while 3 cases lost contact to the donors.In 91 anti-HIV reactive donors , the WB response band type was HIV-1. 10 out of the 29 returned blood donors were allowed to rejoin, 7 of which were considered eli- gible for blood donation.Conclusion The HIV infection type in blood donors in Fuzhou were mainly type 1 ; HIV RNA tests are highly specific to HIV infected samples ; The combination of NAT, ELISA screening tests and reentry strategy would en- sure the donors＇ right to donate while maintain safty requirements in blood donation .

关 键 词：献血者 HIV 核酸检测 WB反应带型 归队 

分 类 号：R446.6[医药卫生—诊断学]