参加国际血型分子免疫血液学实验室室间质评项目结果分析  

Analysis of the results in the external quality assessment schemes in medical laboratories

作　　者：张文静[1] 秦敬民[1] 庄云龙[1] 徐群[1] 

机构地区：[1]山东省血液中心,济南山东250014

出　　处：《中国输血杂志》2017年第8期907-909,共3页Chinese Journal of Blood Transfusion

基　　金：山东省自然科学基金资助(ZR2010HM093)

摘　　要：目的提高实验室分子检测水平,强化实验室人员的质量意识,提高血液检测的安全性和有效性。方法2015年10月至11月参加由德国INSTAND组织的国际医学实验室室间质量评估项目,对发放的未知DNA标本,用PCR-SSP的方法对ABO、Rh、MNS、Kell、Kidd和Duffy等主要血型抗原系统进行基因分型检测。结果对发放的4个DNA标本分别进行抗原的基因分型,结果依次为:61号,B/O1,KEL2/KEL2,JKA/JKB,FYA/FYA,MNs,Ccee,RHD Gen/gene(normal);62号,O1/O1,KEL2/KEL2,JKA/JKB,FYA/FYB,MNs,Ccee,RHD Gen/gene(normal);63号,O1/O1,KEL2/KEL2,JKA/JKB,FYA/FYA,NNs,CCee,RHD Gen/gene(normal);64号,O1/O1,KEL2/KEL2,JKA/JKB,FYB/FYB,MNs,Cc Ee,RHD Gen/gene(normal)。结论鉴定结果与质评反馈结果完全一致。该项目提高了输血医学实验室分子检测的水平,验证了实验室体系的稳定性、保证临床输血检测的安全性和有效性。Objective To improve the performance of molecular detection in the medical laboratory and enhance the safety level and effectiveness of blood tests. Methods Participated the external quality assessment （EQA） schemes in medical laboratories organized by INSTAND and genotyped the main blood groups including ABO, Rh, Kell, Kidd, Duffy and MNS blood group systems using PCR-SSP methods. Results The genotyping results of the four DNA samples are as follows ： no. 61, B/ O1, KEL2/ KEL2 , JKA/ JKB , FYA/ FYA , MNs , Ccee , RI-1D Gen/ gene （normal）; no. 62, O1/O1, KEL2/ KEL2 , JKA/JKB, FYA/FYB, MNs, Ccee, RHD Gen/gene （normal）; no. 63, O1/O1, KEL2/KEL2, JKA/JKB, FYA/FYA, NNs, CCee , RHD Gen/ gene （normal） ; no. 64, 01/01, KEL2/ KEL2 , JKA/ JKB , FYB/ FYB , MNs , CeEe , RHD Gen/ gene （ normal）. Conclusion The genotyping results are in accordance with the feedback results, Participating the EQA would ulti- mately promote the ability of blood molecular detection for the immunohematology laboratories, verify the stability of the ex- perimental system and ensure the safety level and effectiveness of the blood test.

关 键 词：室间质评 血型抗原基因 分子检测 

分 类 号：R457.11[医药卫生—治疗学]