RP-HPLC法测定小儿清肺化痰口服液中苦杏仁苷的含量  

Determination of Amygdalin in Xiaoer Qingfei Huatan Liquid by RP-HPLC

作　　者：易润青 

机构地区：[1]广东省广州市医药职业学校,广州510430

出　　处：《海峡药学》2017年第9期43-45,共3页Strait Pharmaceutical Journal

摘　　要：目的建立RP-HPLC法测定小儿清肺化痰口服液中苦杏仁苷含量的方法。方法采用反相高效液相色谱法,Diamonsil-C18柱,以甲醇-0.1%磷酸水溶液(10∶90,v/v)为流动相,流速为1.0 m L·min-1,检测波长为215nm,柱温为室温。结果苦杏仁苷浓度在4~48μg·m L-1间具有良好的线性关系(r=0.9995),平均回收率为103.4%,方法精密度(RSD)为0.90%(n=6)。结论该法可用于小儿清肺化痰口服液中苦杏仁苷的含量测定。OBJECTIVE To establish an RP-HPLC method for determination of amygdalin in Xiaoer Qingfei Huatan Liquid. METHODS A HPLC method was established. The chromatographic column was Diamonsil-C18. The mobile phase was a mixture of methanol-0. 1% phosphoric acid （10：90 ,v/v）. The flow rate was 1.0mL · min-1 and the detection wavelength was 215nm. RESULTS The linear range of amygdalin was 4 ~ 48μg · mL-1 （r = 0. 9995）. The average recovery for amygdalin was 103.4% ,the precision of the method was 0. 90% （RSD ,n = 6）. CONCLUSION The method can be used for quantitative determination of the preparation.

关 键 词：小儿清肺化痰口服液 苦杏仁苷 反相高效液相色谱法 含量测定 

分 类 号：R927[医药卫生—药学]