寡氧单胞菌中CRISPR位点的分析  

Analysis of CRISPR Site in Stenotrophomonas

作　　者：刘兴雨 毕春霞[2] 辛晓妮[2] 付恒霞 周明艳 王斌 闫志勇 

机构地区：[1]青岛大学医学部微生物学教研室,山东青岛266071 [2]青岛市市立医院,山东青岛266071

出　　处：《微生物学杂志》2017年第4期10-15,共6页Journal of Microbiology

基　　金：山东省优秀中青年科学家科研奖励基金项目(BS2011SW0005);山东省科技攻关基金项目(2007GG3WZ05009)

摘　　要：对寡氧单胞菌基因组中的CRISPR位点进行生物信息学分析。CRISPRdb数据库中公布的和NCBI上下载的共26株寡氧单胞菌的基因组序列,分析其CRISPR位点的分布情况、重复序列、间隔序列以及间隔序列和噬菌体序列数量之间的关系。共发现15个确定的CRISPR结构和132个可疑的CRISPR,不同菌株CRISPR结构中的重复序列具有较强的保守性。间隔序列的靶向基因主要来自细菌的基因组,说明寡氧单胞菌CRISPR的的进化与其他细菌基因有关。此外,间隔序列与前噬菌体数量之间的负相关关系,说明CRISPR能阻止噬菌体的入侵。寡氧单胞菌CRISPR位点的分析为进一步研究耐药性及基因组稳定性奠定了基础。The CRISPR site in Stenotrophomonas genome was analyzed bioinformatically. The relations among the dis- tribution of the CRISPR sites, the repeated sequences, the spaced sequence, and the numbers of phage sequence in 26 strains Stenotrophomonas issued by the CRISPRdb database and downloaded from NCBI were analyzed. Totally 15 CRISPR structures and 132 questionable CRISPRs were found, and the repeated sequences of CRISPR structures in different strains had higher conservativeness. The targeted gene of spaced sequences mainly were from genome of bac- teria, suggested that the evolution of CRISPRs in Stenotrophomonas was relating to the gene of other bacteria. In addi- tion, the negatively correlated to the spaced sequences with the numbers of ante-phage, suggested that CRISPR could block the phages to invade. The analyses of CRISPR sites in Stenotrophomonas provided the basis for further studies on the drug resistance and genome stability.

关 键 词：寡氧单胞菌 CRISPR 重复序列 间隔序列 

分 类 号：Q78[生物学—分子生物学] Q933