多重耐药鲍曼不动杆菌氨基糖苷类修饰酶与16S rRNA甲基化酶基因的研究  

Aminoglycoside Modifying Enzymes and 16S rRNA Methylase Genes in Multi-Drug Resistant Acinetobacter baumannii

作　　者：杨艳 王厚照 张玲 

机构地区：[1]厦门大学附属成功医院/安徽医科大学解放军174临床学院,福建厦门361003

出　　处：《微生物学杂志》2017年第4期28-33,共6页Journal of Microbiology

基　　金：全军医学科技青年培育项目(13QNP047)

摘　　要：了解氨基糖苷类修饰酶、16S rRNA甲基化酶基因在多重耐药鲍曼不动杆菌中的流行情况。收集2014年12月至2015年3月厦门大学附属成功医院住院患者临床分离的多重耐药鲍曼不动杆菌共28株,采用VIKET Compact 2全自动细菌鉴定系统进行细菌鉴定,应用纸片扩散法(K-B法)检测鲍曼不动杆菌对抗菌药物的耐药性,采用聚合酶链反应(PCR)法检测氨基糖苷类修饰酶、16S rRNA甲基化酶基因。结果显示,多重耐药鲍曼不动杆菌除对头孢哌酮/舒巴坦耐药率为21.4%外,对其他所测药物耐药率均>50%,本组28株多重耐药鲍曼不动杆菌共检出5种氨基糖苷类修饰酶基因aac(3)-Ⅰ、aac(3)-Ⅱ、aac(6')-Ⅰb、ant(3")-Ⅰ、aph(3')-Ⅰ和1种16S rRNA甲基化酶基因arm A,阳性率分别为85.7%(24株)、7.14%(2株)、67.8%(19株)、92.9%(26株)、53.6%(15株)和82.1%(23株)。氨基糖苷类修饰酶、16S rRNA甲基化酶耐药基因是多重耐药鲍曼不动杆菌对氨基糖苷类耐药的重要原因。The prevalence of aminoglycoside modifying enzymes and 16S rRNA methylase genes in multi-drug resist- ant Acinetobacter baumannii was studied. A total of 28 muhi-drug resistant A. baumannii isolates were collected from Dec. 2014 to Mar. 2015 from patients in the ChengGong Hospital Affiliated to Xiamen University. Bacterial identifi- cation was performed by VITEK 2 Compact automatic bacterial identification system. Kirby-Bauer Diffusion Method was used to detect the drug resistance of antimicrobial drugs. The aminoglycoside modifying enzymes and 16S rRNA methylase genes were amplified by polymerase chain reaction （PCR）. The results showed that the resistance rate of all the other measured drugs were all above 50% except for cefoperazone-sulbactam （21.4%）. 5 kinds of aminoglycoside modifying enzyme genes including aac （ 3 ） -I, aac （ 3 ） -II, aac （ 6＇ ） -Ib, ant （ 3＂ ） -I, aph （ 3＇ ） -I and one kind of 16S rRNA methylase gene armA were found in 28 strains of multi-drug resistant A. baumannii with the positive rates at 85.7% （24 strains）, 7.14% （2 strains）, 67.8% （ 19 strains）, 92.9% （26 strains） , 53.6% （ 15 strains） and 82.1% （23 strains） respectively. Therefore the genes of aminoglyeoside modifying enzymes and 16S rRNA methylase were the major reasons for the resistance to aminoglycosides in multi-drug resistant A. baumannii.

关 键 词：鲍曼不动杆菌 多重耐药性 氨基糖苷类修饰酶 16S rRNA甲基化酶 耐药基因 

分 类 号：Q939.93[生物学—微生物学]