生合成途径特异性转录因子及其激活真菌沉默次级代谢产物的研究进展  

Research progress on the pathway-specific transcription factors and their application in activating cryptic fungal secondary metabolites

作　　者：崔小清 丁壮[1] 武莉 车茜[1] 李德海[1] 顾谦群[1] 朱天骄[1] CUI Xiao-qing DING Zhuang WU Li CHE Qian LI De-hai GU Qian-qun ZHU Tian-jiao(Key Laboratory of Marine Drugs, Chinese Ministry of Education, School of Medicine and Pharmacy, Ocean University of China, Qingdao 266003, Chin)

机构地区：[1]中国海洋大学海洋药物教育部重点实验室,医药学院,山东青岛266003

出　　处：《中国海洋药物》2017年第4期75-82,共8页Chinese Journal of Marine Drugs

基　　金：国家自然科学基金--山东联合基金项目(U1406402);国家自然科学基金项目(21542001)资助

摘　　要：真菌次级代谢产物一直以来都是现代天然药物研究的重要资源,如何应用新技术和新方法高效快速地从真菌中发现更多活性独特的新颖次级代谢产物,一直是该领域研究的热点。近几年,真菌全基因组测序和生物信息学分析表明,真菌基因组中包含大量处于"沉默"(silent或cryptic)状态的次级代谢基因簇,这意味着真菌基因组中还有丰富的次级代谢产物生物合成潜能有待于激活和挖掘。生合成途径特异性转录因子(pathway-specific transcription factor)能够激活和调控真菌"沉默"次级代谢产物基因簇的转录表达,促进相应代谢产物的生物合成,从而提高新代谢产物的发现几率,已成为挖掘真菌沉默基因簇、激活菌株代谢潜能的有效手段。本文对目前生合成途径特异性转录因子及其激活真菌次级代谢产物的研究进展进行综述。Fungal secondary metabolites have always been an important source of natural derived drugs. In recent years, fungal whole-genome sequencing and bioinformaties analysis revealed that plenty of secondary metabolites biosynthesis genes were still not sufficiently researched. How to activate these cryptic gene clusters and discover the metabolites is research hot spot in the world. Pathway-specific transcription factor could active and regulate the expression of these cryptic biosynthetic genes which makes the discovery of new metabolites. Therefore, overexpression the pathway-specific transcription factor has now become an effective method of fungus genome mining for secondary metabolites. In this paper, research progress of pathway-specific transcription factor and their applying in activating cryptic fungal secondary metabolites were reviewed.

关 键 词：真菌 次级代谢产物 代谢潜能 途径特异性转录因子 沉默基因簇 

分 类 号：R931[医药卫生—生药学]