Insights into the plastid diversity of Daphne blagayana Freyer （Thymelaeaceae）  

作　　者：Ziva Fiser Pecnikar Natasa Fujs Robert Brus Dalibor Ballian Elena Buzan 

机构地区：[1]Department of Biodiversity, Faculty of Mathematics, Natural Sciences and Information Technologies, University of Primorska, Glagoliaska 8,6000 Koper, Slovenia [2]Department of Forestry and Renewable Forest Resources, Biotechnical Faculty, University of Ljubliana, Vecna pot 83,1000 Ljubliana, Slovenia [3]Faculty of Forestry, University of Sarajevo, Zagrebacka 20, 71000 Sarajevo, Bosnia and Herzegovina [4]Slovenian Forestry Institute, Vecna pot 2, 1000 Ljubljana, Slovenia

出　　处：《Journal of Systematics and Evolution》2017年第5期437-445,共9页植物分类学报（英文版）

摘　　要：Daphne blagayana is a small decumbent bush from the family Thymelaeaceae with a fragmented distribution range, comprising the Balkan Peninsula and the southern Romanian Carpathians. Due to its rarity it is protected by national legislation in almost all countries within its range. The aim of this work was to assess the plastid diversity of Daphne blagayana from 21 locations throughout its distribution range using the chloroplast intergenic regions psbA-trnH and trnK-matK with the gene matK. Moreover, we tried to determine with DNA and morphometric characters whether the division of the species into subspecies and varieties, which were mentioned in some literature （subsp. lerchenfeldiana, var. kellereri, subsp, zogovi^ii）, reflects its phylogeographic structure. The results suggest the existence of three clusters： the northwestern cluster （ESUO, including Slovenian and Italian populations, the southern cluster （ESU2）, including populations from Macedonia, Montenegro and some populations from southern Bosnia and Herzegovina, and the central cluster, including the remaining populations （Bulgaria, Romania and rest of the populations from Bosnia and Herzegovina）. The results of the genetic as well as morphometric study do not support the existence of subsp, lerchenfeldiana and vat. kellereri as distinct infraspecific categories of D. blagayana, thus we suggest that ESU＇s should be used instead in planning conservation actions.Daphne blagayana is a small decumbent bush from the family Thymelaeaceae with a fragmented distribution range, comprising the Balkan Peninsula and the southern Romanian Carpathians. Due to its rarity it is protected by national legislation in almost all countries within its range. The aim of this work was to assess the plastid diversity of Daphne blagayana from 21 locations throughout its distribution range using the chloroplast intergenic regions psbA-trnH and trnK-matK with the gene matK. Moreover, we tried to determine with DNA and morphometric characters whether the division of the species into subspecies and varieties, which were mentioned in some literature （subsp. lerchenfeldiana, var. kellereri, subsp, zogovi^ii）, reflects its phylogeographic structure. The results suggest the existence of three clusters： the northwestern cluster （ESUO, including Slovenian and Italian populations, the southern cluster （ESU2）, including populations from Macedonia, Montenegro and some populations from southern Bosnia and Herzegovina, and the central cluster, including the remaining populations （Bulgaria, Romania and rest of the populations from Bosnia and Herzegovina）. The results of the genetic as well as morphometric study do not support the existence of subsp, lerchenfeldiana and vat. kellereri as distinct infraspecific categories of D. blagayana, thus we suggest that ESU＇s should be used instead in planning conservation actions.

关 键 词：Balkan Peninsula conservation genetics CPDNA endemic plants genetic structure refugia. 

分 类 号：Q959.7[生物学—动物学] Q949.761.1