严重烧伤患者早期血清微小RNA差异表达及其信号通路分析  

Differential expression of microRNAs in serum of severe burn patients and analysis of the signaling pathway at early stage

作　　者：王姗 阮琼芳 谢卫国 陈斓 蒋梅君 阮晶晶 叶子青 

机构地区：[1]武汉大学中南医院普外科,430071 [2]武汉大学同仁医院暨武汉市第三医院烧伤研究所

出　　处：《中华烧伤杂志》2017年第10期639-643,共5页Chinese Journal of Burns

基　　金：国家自然科学基金青年科学基金（81501667）;湖北省自然科学基金（2016CFB473）;武汉市卫生和计划生育委员会科研项目重点项目（WX17A03）

摘　　要：目的探讨严重烧伤患者早期血清微小RNA的差异表达及其信号通路。方法选择武汉大学同仁医院暨武汉市第三医院2015年7月收治的符合入选标准的3例严重烧伤患者，另选择3名健康成年志愿者。于烧伤患者伤后24—48h，烧伤患者和健康志愿者各取静脉全血6mL并分为烧伤组和健康对照组。2mL全血用于检测白细胞计数、中性粒细胞含量。4mL全血分离血清，一半血清用于检测血糖、总蛋白及白蛋白含量；一半血清采用Trizol法提取总RNA，利用微小RNA芯片技术筛选2组间差异表达比值大于或等于1．500的微小RNA，对差异表达微小RNA进行聚类分析、京都基因和基因组百科全书（KEGG）信号通路功能富集分析。对数据行t检验。结果（1）烧伤组全血白细胞计数、中性粒细胞及血清血糖含量均显著高于健康对照组（t值为4．27～7．83，P〈0．05或P〈0．01），烧伤组血清总蛋白和白蛋白含量均显著低于健康对照组（t值分别为-12．80、-12．36，P值均小于0．01）。（2）与健康对照组比较，烧伤组血清差异表达比值大于1．500的微小RNA共48个，其中表达上调的共22个，表达下调的共26个。烧伤组血清微小RNA表达谱与健康对照组不同。（3）KEGG信号通路功能富集分析显示，与健康对照组比较，烧伤组血清差异表达的微小RNA参与肿瘤转录失调信号通路、肿瘤蛋白聚糖信号通路、长时程增强作用信号通路、肿瘤相关微小RNA信号通路、柠檬酸循环信号通路、TNF信号通路、黏着斑信号通路、内吞作用信号通路、胰岛素分泌信号通路、雌激素信号通路。结论严重烧伤患者血清微小RNA表达谱与健康人不同，其差异表达的微小RNA可能参与严重烧伤早期能量代谢、炎症反应、血糖调节等重要的病理生理过程。Objective To explore differential expression of microRNAs in serum of patients with severe burn and analysis of the signaling pathway at early stage. Methods In this study, we included three healthy adult volunteers and three patients with severe burn, conforming to the inclusion criteria and hospitalized in Tongren Hospital of Wuhan University ＆ Wuhan Third Hospital in July 2015. Venous whole blood of 6 mL of each burn patient and healthy volunteer was collected at 24 to 48 h post injury of burn patients. The whole blood was divided into burn group and healthy control group. Whole blood of 2 mL of each one was used to determine white blood cell count and neutrophile granulocyte content. Serum was separated from the other whole blood of 4 mL of each one. Half of serum was used to determine content of blood glucose, total protein, and albumin; another half of serum was used to extract total RNA with Trizol method. The differentially expressed mieroRNA, with differential expression ratio larger than or equal to 1. 500 between 2 groups, were screened by microRNA chip technique. Then cluster analysis and functional enrichment analysis of Kyoto encyclopedia of genes and genomes （KEGG） signaling pathway were performed on the differentially expressed microRNAs. Data were processed with t test. Results （1） Content of white blood cell count,neutrophile granulocyte of whole blood, and blood glucose of serum of patients in burn group was obviously higher than that in healthy control group （ with t values from 4.27 to 7.83, P 〈 0.05 or P 〈 0.01 ）. Content of total protein and albumin of serum of patients in burn group was significantly lower than that in healthy control group （ with t values respectively - 12.80 and - 12.36, P values below 0.01 ）. （2） Compared with those in serum of healthy control group, differential expression ratios of 48 microRNAs in serum of burn group were larger than 1. 500, with 22 up-regulated microRNAs and 26 down-regulated microRNAs. MicroRNA expression profile in serum o

关 键 词：烧伤 血清 基因表达谱 微小RNA 

分 类 号：R644[医药卫生—外科学]