启膈方对人食管癌细胞Eca109、TE13及Cx26表达的影响  

Effect of Qige Fang (启膈方) on Expression of Eca109,TE13 and Cx26 in Human Esophageal Carcinoma Cells

作　　者：史会娟[1] 石冬璇 李丽[3] 常青鲜 李晶[1] SHI Huijuan SHI Dongxuan LI Li CHANG Qingxian LI Jing(Fourth Hospital of Hebei Medical University, Shijiazhuang, 050011 Hebei Medical University Hebei General Hospital Shijiazhuang Yiling Pharmaceutical Co. ,Ltd)

机构地区：[1]河北医科大学第四医院,河北省石家庄市健康路12号050011 [2]河北医科大学 [3]河北省人民医院 [4]石家庄以岭药业股份有限公司

出　　处：《中医杂志》2017年第19期1671-1675,共5页Journal of Traditional Chinese Medicine

基　　金：国家自然科学基金(81403310);河北省科技计划项目(13277718D)

摘　　要：目的探讨启膈方治疗食管癌的可能作用机制。方法采用人食管癌细胞Eca109及TE13,将各细胞分为对照组及启膈方高剂量组、启膈方低剂量组。采用MTT法观察启膈方对食管癌细胞增殖的影响,应用Transwell小室法检测启膈方对食管癌细胞迁移的影响,应用激光共聚焦显微镜及流式细胞术观察启膈方对食管癌细胞间隙连接蛋白26(Cx26)表达的影响,采用RT q-PCR法观察启膈方对食管癌细胞中Cx26水平的影响。结果启膈方正常剂量在体外环境不能抑制Eca109及TE13细胞增殖。启膈方高剂量组Eca109迁移细胞数明显少于对照组和启隔方低剂量组(P<0.01),启膈方高、低剂量组TE13迁移细胞数明显少于对照组,且启膈方高剂量组明显少于启隔方低剂量组(P<0.05或P<0.01)。经启膈方作用后的食管癌细胞Eca109和TE13中Cx26蛋白及mRNA水平表达明显增高(P<0.05)。结论启膈方可抑制人食管癌细胞Eca109、TE13的增殖与迁移,并能够增强Cx26在蛋白水平及转录水平的表达,使食管癌细胞间隙连接增强,从而抑制细胞的迁移。这可能是其治疗食管癌的部分作用机制。Objective To explore the potential mechanism of Qige Fang （启膈方） in treating esophageal carcinoma.Methods Human esophageal cancer cells Eca109 and TE13 were used and divided into the control group,the high-dose Qige group and the low-dose Qige group.MTT method was used to observe the effect of Qige Fang on the proliferation of esophageal cancer cells; Transwell chamber method was used to detect the effect of Qige Fang on the migration of esophageal cancer cells; the effect of Qige Fang on the expression of connexin 26 （Cx26） in esophageal cancer cells was observed by laser confocal microscopy and flow cytometry; the effect of Qige Fang on the expression of Cx26 mRNA in esophageal cancer cells was observed by RT q-PCR.Results The normal dose of Qige Fang cannot inhibit the proliferation of Eca109 and TE13 cells in vitro.The number of Eca109 migrating cells in the high dose group was significantly lower than that in the control group and the low dose group （P 〈 0.01）.The numbers of TE13 migrating cells in the high and low dose groups were significantly lower than that in the control group,and that in the high dose group was significantly lower than that in the low dose group （P 〈 0.05 or P 〈 0.01）.The expressions of Cx26 protein and mRNA in esophageal cancer cells Eca109 and TE13 significantly increased after the administration of Qige Fang （P 〈 0.05）.Conclusion Qige Fang can inhibit the proliferation and migration of human esophageal cancer cells Eca109 and TE13,and can enhance the expression of Cx26 at protein level and transcription level,and increase the gap junction of esophageal cancer cells,thus inhibiting cell migration.This might be part of the mechanism of Qiga Fang in treating esophageal carcinoma.

关 键 词：食管癌 启膈方 细胞间隙连接蛋白 细胞迁移 

分 类 号：R285[医药卫生—中药学]