真菌诱导子对南方红豆杉细胞生长及紫杉醇合成的影响  

Effects of Fungus Elicitor on the Cells Growth and Paclitaxel Accumulation of Taxus chinensis var. mairer

作　　者：徐志荣 赵英杰 王婷 魏赛金 

机构地区：[1]江西农业大学生物科学与工程学院/江西省农业微生物资源开发与利用工程实验室,南昌330045

出　　处：《湖北农业科学》2017年第17期3283-3286,共4页Hubei Agricultural Sciences

基　　金：江西省科技计划项目(20133BBG70090)

摘　　要：用真菌诱导子处理南方红豆杉(Taxus chinensis var.mairer)悬浮细胞,测定细胞生长指数、紫杉醇含量及其细胞酶活的变化,初步探讨真菌诱导子对南方红豆杉悬浮细胞生长的影响。结果表明,在细胞悬浮培养第8天加入100μg/m L真菌诱导子,可明显增加悬浮细胞的生物量,紫杉醇的含量也有提高;添加真菌诱导子能抑制细胞多酚氧化酶(PPO)和苯丙氨酸解氨酶(PAL)的活性,有利于细胞生长。Taxus chinensis var. mairer suspension cells were treated with fungal elicitor. The effects of fungal elicitors on the growth of T. chinensis var. mairer suspension cells were studied to measure the changes of cell growth index, paclitaxel content and enzyme activity. The results showed that, when the elicitors were added into the cell cultures at the 8th day, 100μg/m L fungus elicitor had promoted on the cell growth and the synthesis of paclitaxel; The addition of fungus elicitor promoter inhibited the activity of polyphenol oxidase（PPO） and phenylalanine ammonia（PAL）, and it is good for cell growth.

关 键 词：南方红豆杉(Taxus CHINENSIS var.mairer) 细胞悬浮培养 真菌诱导子 紫杉醇 

分 类 号：S791.49[农业科学—林木遗传育种] Q813[农业科学—林学]