跑台运动对PD模型大鼠运动皮层异常β振荡的调节作用  

Treadmill Exercise Modulates Abnormal β Oscillation of M1 in the Hemiparkinsonian Rat

作　　者：时凯旋 刘晓莉[1] 乔德才[1] 林湘明[1] 成雅鑫 张凌韬 SHI Kaixuan LIU Xiaoli QIAO Decai LIN Xiangming CHENG Yaxin ZHANG Lingtao(College of PE and Sports, Beijing Normal University, Beijing 100875 ,China)

机构地区：[1]北京师范大学体育与运动学院,北京100875

出　　处：《西安体育学院学报》2017年第5期590-598,共9页Journal of Xi'an Physical Education University

基　　金：国家自然科学基金项目(31571221);北京市自然科学基金项目(5142012)

摘　　要：目的观察运动对帕金森病(parkinson's disease,PD)模型大鼠初级运动皮层(M1)异常β振荡的调节作用,探究运动干预改善PD行为功能的可能原因。方法清洁级SD大鼠18只分为3组,即假手术组(Control,n=5)、PD组(PD,n=6)和PD运动组(PD+Ex,n=7),于内侧前脑束注射6-羟基多巴(6-OHDA)同时于M1区植入多通道电极。大鼠在造模后第1周颈部皮下注射阿朴吗啡(APO)旋转行为实验评价模型成功;PD+Ex组在第1周开始进行跑台训练(11 m/min,30 min/d,5 d/周,4周),各组大鼠在第0/1/2/3/4采用自主错步仪进行行为测试;采用在体多通道电生理技术,观察各组大鼠M1区在第0/1/2/3/4的局部场电位(LFPs),免疫组化技术检测黑质和纹状体内酪氨酸羟化酶(TH)表达。结果 TH免疫组化结果显示,PD+Ex组较PD组无显著性改变(P>0.05);自主错步行为测试结果发现,PD+Ex组的通过时间、前肢错步数和尾部滑落数及潜伏期较PD组均有显著性改善(P<0.01)。电生理学结果显示,PD组较Control组10~30 Hz的β频段异常增高(P<0.01);PD+Ex组在第1周β振荡的PSD较第0周无显著改变(P>0.05),在第2/3/4周(P<0.05)显著降低。结论运动干预调节运动皮层的功能活性,抑制M1区异常β振荡,进而改善其步态及自主活动能力。推测运动降低PD模型大鼠M1区异常β振荡介导了皮层-纹状体Glu能突触可塑性,调控输入至基底神经节的运动编码信息,最终改善大鼠的行为功能。Objective To study the beta oscillation changes and its electrophysiological associations with exercise＇s effects in parkinson＇s disease,we investigate local field potentials of M1 in hemiparkinsonian rats. Methods Eighteen adult male SD rats were randomly divided into four groups： sham-operation group（ n = 5）,animal model group（ n = 6） and animal with exercise group（ n = 7）. We used 6-OHDA injection in medial forebrain bundle in the right brain of SD rat to establish the parkinsonian rat model and the multi-electrode arrays was implanted into primary motor cortex to record local field potentials,apomorphine-induced rotational test was performed at 1 week after 6-OHDA injection as model evaluations. At 3 weeks after 6-OHDA injection,the animals in the exercise group were forced to run on a motorized treadmill（ 11 m/min,30 min/d,5 d/w,4 weeks）. Automatic foot faults test were carried out at 0/1/2/3/4 weekend; we used in vivo multichannel recording technology to record local field potentials in the primary motor cortex of freely moving parkinsonian rats. TH-immunohistochemistry technology was used to detect dopamine axonal fibers in the striatum and dopamine neurons in the substantia nigra pars reticulata. Results Anatomical examination indicates that compared to the PD rats,PD＋Ex rats had no significant loss of dopamine axonal fibers in the striatum and dopamine neurons in the substantia nigra pars reticulata（ P〈0. 05）. Behavioral test results indicates that six weeks after surgery,total crossing time/front leg errors/tail errors/input latency time lower in PD＋Ex rats than in PD rats（ P〈0. 01）. Electrophysiological results indicated that power spectral density of β band was higher in PD rats than Control rats（ P〈0. 01）,while PD＋Ex rats had reduced the abnormal PSD of β band than PD rats（ P〈0. 05）. In PD＋Ex rats,PSD of β band had no significant change at the 1 week compared with the 0 week（ P〈0. 05）,but lower at the2/3/4 week than the 0 week（ P〈0. 0

关 键 词：跑台运动 帕金森病 运动皮层 局部场电位 β振荡 

分 类 号：G804.7[文化科学—运动人体科学]