单粒精米的DNA磁珠法高效率提取技术研究  

High Efficient DNA Extraction Technology from Single Milled Rice Grain by Magnetic Bead Methods

作　　者：寇姝燕[1] 邹茜[1] 朱振华[1] 刘慰华[1] 苏振喜[1] 赵国珍[1] 蒋聪[2] 袁平荣[1] 

机构地区：[1]云南省农业科学院粮食作物研究所,云南昆明650205 [2]云南省农业科学院生物技术与种质资源研究所,云南昆明650223

出　　处：《西南农业学报》2017年第9期1981-1985,共5页Southwest China Journal of Agricultural Sciences

基　　金：云南省科技计划生物种业重大专项(2015ZA003);云岭产业技术领军人才项目;云南省现代农业水稻产业技术体系;中韩水稻合作研究项目

摘　　要：【目的】文章研究了利用磁珠法高效率提取单粒精米DNA的技术方法。【方法】以4份市售大米为材料,利用磁珠吸附法从单粒精米样品中提取基因组DNA并进行PCR扩增,进行提取效率的检测。【结果】提取的20份基因组DNA条带整齐、无弥散、质量高,浓度均在1 ng/μl以上;进一步,以提取液为模板,利用SSR引物RM247和RM282进行PCR扩增,电泳检测后分别得到180和120 bp的目标条带。【结论】利用磁珠法提取的单粒精米DNA可以直接用于PCR反应;结果为特殊目的或稀缺材料的微量DNA提取检测提供技术支持。[ Objective ] A high efficient DNA extraction technology by magnetic bead methods from single milled rice grain was studied in thisreport. [ Methad]4 milled rice samples were used to carry out genomic DNA extraction through magnetic bead methods and PCR processingto check the efficient of extraction results. [ Result] Twenty single milled rice grain DNA were extracted through magnetic bead methods. Af-ter electrephoresis checking, the result showed high quality of genomic DNA bands without diffusion, and the DNA concentrations above Ing/~L1. Moreover, 2 SSR markers, RM247 and RM282, were used to carry out PCR amplified with the extraction DNA solution as templatesdirectly. And two bands with 180 and 120 bp were detected respectively as anticipated. [ Conclusion] The research results demonstrated afairly good quality of the DNA extracting, which also supported a feasibility and high-efficient method to extracted trace DNA from singlemilled rice grain.

关 键 词：单粒精米 水稻DNA 磁珠法 提取 

分 类 号：S511[农业科学—作物学]