氧化锆氧化铝陶瓷复合材料对人牙周膜细胞增殖、凋亡和骨诱导活性的影响  

Effects of zirconia/alumina ceramic composite on proliferation, apoptosis and bone induction activity of human periodontal ligament cells

作　　者：王佳宁[1] 

机构地区：[1]白城医学高等专科学校,吉林省白城市137000

出　　处：《中国组织工程研究》2017年第26期4155-4159,共5页Chinese Journal of Tissue Engineering Research

基　　金：吉林省教育厅"十三五"社会科学研究项目(吉教科文合字[2016]第30号):利用TDS系统对口腔修复教学的~~

摘　　要：背景:氧化锆氧化铝陶瓷复合材料具有优良的抗腐蚀性、较高的强度和韧性,具有广泛的应用前景。目的:探究氧化锆氧化铝陶瓷复合材料对人牙周膜细胞增殖、凋亡和骨诱导活性的影响。方法:将第3-5代人牙周膜细胞分2组培养,对照组细胞常规培养,实验组与氧化锆氧化铝陶瓷复合材料共培养,培养1-5 d,MTT法检测细胞增殖,Tunel法检测细胞凋亡,免疫组织化学法检测细胞Ⅰ型胶原蛋白表达,碱性磷酸酶染色检测细胞成骨活性。结果与结论:培养1-5 d内,两组细胞增殖、凋亡、碱性磷酸酶活性比较差异均无显著性意义;培养5 d时,两组Ⅰ型胶原表达比较差异无显著性意义;结果表明,氧化锆氧化铝陶瓷复合材料对牙周膜细胞增殖、凋亡和骨诱导活性均无影响。BACKGROUND： Zirconia/alumina ceramic composite with excellent corrosion resistance, high strength and toughness has wide application prospect.OBJECTIVE： To investigate the effect of zirconia/alumina ceramic composite on proliferation, apoptosis and bone induction activity of human periodontal ligament cells.METHODS： Human periodontal ligament cells at passages 3-5 were cultured routinely as control group and co-cultured with zirconia/alumina ceramic composite as experimental group. After 1-5 days of culture, MTT,TUNEL, immunohistochemical detection, and alkaline phosphatase staining were used to detect cell proliferation,apoptosis, type I collagen expression and osteogenic activity, respectively.RESULTS AND CONCLUSION： Within 1-5 days of culture, there were no differences in cell proliferation, apoptosis and alkaline phosphatase activity between the two groups. At 5 days of culture, the type I collagen expression showed no difference between the two groups. Taken together, the zirconia/alumina ceramic composite has no effect on the proliferation, apoptosis and bone induction activity of human periodontal ligament cells.

关 键 词：生物材料 口腔生物材料 氧化锆氧化铝陶瓷复合材料 牙周膜细胞 生物相容性 

分 类 号：R318[医药卫生—生物医学工程]